| Purpose: To observe the effect of Yiqi Huoxue Tongluo Decoction on blood sugar,sciatic nerve conduction velocity,pain threshold and pathological morphology of sciatic nerve in diabetic rats,and to observe the effect of Yiqi Huoxue Tongluo Decoction on serum TNF-α,IL-6,Na+-K+-ATPase in diabetic rats,and to explore the therapeutic mechanism of Yiqi Huoxue Tongluo Decoction,so as to provide a deep theoretical basis for the diagnosis and treatment of diabetes with traditional Chinese and western medicine.Materials and methods: 60 male wistar rats of SPF grade,10 rats in the normal group were randomly selected,and the remaining 50 rats were used to establish diabetic rat models.Streptozotocin(53mg/kg)was injected intraperitoneally,and the blood sugar of the rats was measured to be ≥16.7mmol/L after 2 mice were established.Excluding 5 rats that were not successfully established,a total of 45 rats were successfully established,and the 45 rats that were successfully established were divided into model groups.Both the normal group and the model group were gavaged with distilled water,the treatment group was gavaged with Yiqi Huoxue Tongluo recipe(16.2ml/kg/d),and the western medicine group was gavaged with Mecobalamin suspension(10.8ml/kg/d)for 8 weeks.Observation indexes: blood sugar,body weight,motor nerve conduction velocity,pain threshold and pathological changes of sciatic nerve in rats in each group.Serum TNF-α,IL-6 and Na+-K+-ATPase concentrations in DPN rats were detected by ELISA.All statistical methods are expressed by S,and the statistical software SPSS23.0 is used to test the one-way ANOVA,and P<0.05 shows that there is a statistical difference.Use TCM systematic pharmacology database analysis platform(TCMSP)to find the components and related targets of Yiqi Huoxue Tongluo recipe,collect the target information related to DPN in four databases:Gene Cards,OMIM,TTD and Dis Ge NET,and screen out the intersection targets of drugs and diseases,draw the "PPI" network diagram of intersection targets through String database and Cytoscape3.8.0 software,and screen out the intersection core targets of drugs and diseases;Through the Metascape database,the targets of interaction intersection were enriched and analyzed,and the pathways with high correlation between enrichment degree and DPN process were selected,so as to explore the mechanism of Yiqi Huoxue Tongluo Decoction in treating DPN.Results:1.After intraperitoneal injection of 2%STZ,5 rats were excluded,and the remaining 45 rats were successfully established.Four rats died during 8 weeks of drug intervention,and 41 rats finally survived,including 10 rats in normal group,13 rats in model group,14 rats in traditional Chinese medicine group and 14 rats in western medicine group.During the experiment,the rats in the model group were overeating,drinking,urinating,emaciated and dull.The state of rats in other groups is obviously better than that in the model group.The gradual weight loss of rats in the model group is the most obvious,and the weight gain of rats in the normal group is the most stable.The weight gain of rats in Chinese medicine group and western medicine group is significant(P<0.01),and the weight gain of rats in Chinese medicine group is higher than that in western medicine group(P<0.05).2.The blood sugar of rats in each treatment group showed a downward trend(P < 0.01).Among them,the trend of blood sugar reduction in western medicine group and Chinese medicine group was significantly better than that in model group.3.The mechanical pain threshold of rats in normal group was higher than that in treatment group(P<0.01).After drug intervention,the mechanical pain threshold of rats in each treatment group showed an obvious upward trend compared with the model group(P<0.01),and the improvement of Chinese medicine group was better than that of western medicine group.4.The conduction velocity of motor nerve in normal group was significantly higher than that in model group and treatment group(P<0.01).During the a DPNinistration,the conduction velocity of rats in each treatment group showed a significant upward trend compared with that in the model group(P<0.01).5.The results of HE staining showed that the cells in the slices of the model group were irregularly arranged,and the nerve tissue structure was not complete.After treatment,both the western medicine group and the traditional Chinese medicine group improved significantly,and the improvement degree of the traditional Chinese medicine group was better than that of the western medicine group.6.The results of 6.ELISA showed that compared with the normal group,the serum Na+-K+-ATPase activity in the model group was significantly decreased(P<0.01),while the serum Na+-K+-ATPase activity in the western medicine group and the Chinese medicine group was significantly increased compared with the model group,and the serum Na+-K-ATPase activity in the Chinese medicine group was higher than that in the western medicine group(P<0.01).Compared with the normal group,the concentrations of IL-6 and TNF-α in the serum of rats in the model group increased significantly(P<0.01),while those in the Chinese medicine group decreased significantly(P<0.01).7.The results of network pharmacology show that Yiqi Huoxue Tongluo recipe can achieve the therapeutic effect of treating diabetic peripheral neuropathy by regulating key targets such as NFKBIA,MAPK1TP53,TNF and signal pathways such as AGE-RAGE.Conclusion:1.Yiqi Huoxue Tongluo recipe can reduce blood sugar,control weight loss,reduce the latency of pain threshold in DPN rats,increase the conduction velocity of motor nerve in DPN rats,improve the pathological morphology of sciatic nerve in DPN rats,and alleviate the pathological damage of sciatic nerve tissue in DPN rats.2.Yiqi Huoxue Tongluo recipe can reduce the levels of TNF-α and IL-6 in DPN rats,inhibit inflammatory reaction,and at the same time increase the activity of Na+-K+-ATPase in DNP rats,thus improving nerve conduction speed and improving nerve injury.3.The results of network pharmacology show that Yiqi Huoxue Tongluo recipe can achieve the therapeutic effect of treating diabetic peripheral neuropathy by regulating signal pathways such as AGE-RAGE and regulating inflammatory response and oxidative stress response. |
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