Study On The Characteristics Of Urine Metabolomics In Children With Spleen Deficiency And The Effect Of Liu's Pediatric Massage On The

Objective: Comparing the differences in metabolomics between children with spleen deficiency and healthy children in their urine samples,observing the effects of Xiangxi Liu’s pediatric tuina on the constitution and urine metabolomics of children with spleen deficiency,and exploring and enriching the theoretical system of Xiangxi Liu’s pediatric tuina.Methods: Twenty children with spleen deficiency were included,and Liu’s pediatric tuina was used to treat the children with spleen deficiency.Urine samples were collected before and after the intervention and subjected to metabolomics analysis.Seventeen healthy children were included to analyze their urinary metabolomics characteristics and compared with those of children with spleen deficiency.Symptom scores of children were recorded before and after tuina,and the efficacy of pediatric tuina on children with spleen deficiency was observed.The samples were detected by ultra-high performance liquid chromatography-mass spectrometry.Multivariate statistical analysis was used to process the obtained data,and then the differential metabolites and metabolic pathways were compared between groups.Results:1.In the intervention of Xiangxi Liu’s pediatric tuina for children with spleen deficiency,there were no cases of complete recovery,one case of significant improvement,thirteen cases of effectiveness,and six cases of ineffectiveness,with a total effective rate of 70%.After the treatment of Xiangxi Liu’s pediatric tuina,the score of feces,appetite,complexion,and total score of children with spleen deficiency improved significantly(P<0.05).Sleep did not improve significantly(P>0.05).2.Comparison of urine metabolism between children with spleen deficiency and healthy group:differential metabolites were L-homocysteine,naringin-3-O-glucoside(an increasing trend),and riboflavin,cyclic adenosine monophosphate,and valine(a decreasing trend);the representative differential metabolic pathway between the two groups was cysteine and methionine metabolism,tryptophan metabolism,and arginine and proline metabolism.3.Comparison of urine metabolism before and after the intervention of Liu’s pediatric tuina for children with spleen deficiency: differential metabolites were S-adenosyl-L-homocysteine,L-methionine,adenosine,and oleic acid(a decreasing trend),and cholesterol(an upward trend).The representative differential metabolic pathway between the two groups was taurine and hypo-taurine metabolism,alanine,aspartate,and glutamate metabolism,and cysteine and methionine metabolism.Conclusion:1.The internal mechanism of pediatric spleen deficiency may be related to metabolic pathways such as cysteine and methionine metabolism,and the dietary preferences of children with spleen deficiency may be one of the important factors leading to spleen deficiency.2.The treatment of Xiangxi Liu’s pediatric tuina on children’s spleen-deficiency constitution is feasible and clinically effective.3.Xiangxi Liu’s pediatric tuina can affect the metabolic status of children with spleen deficiency,and its internal mechanism for intervening in the pediatric spleen-deficiency constitution may be related to metabolic pathways such as taurine and cysteic acid metabolism.