Effects Of Qufengmingmu Pills On TLR4/MyD88/NF-κB Pathway Proteins In Rats With Experimental Autoimmune Uveiti

Objective:By establishing IRBP to induce the experimental autoimmune uveitis(EAU)rats model,observe the effect of Qufeng Mingmu Pill on the expression of TLR4,My D88,NF-κB p65 on the EAU of retina on rats,explore the therapeutic effect and mechanism of Qufeng Mingmu Pill on EAU.Methods: 70 SPF pure male Lewis rats were divided into a blank group(19rats)and a model(51rats)according to random digital table method.The model was established under the IRBP induction to establish an EAU rat model.After immunization,the ocular inflammatory reaction was observed under slit lamp microscope every day,and the inflammation score was evaluated according to the Caspi scoring standard.On the 7th day after modeling,3 rats were randomly selected from the blank control group and the model group and killed.The eyeballs were taken for pathological section and histopathological examination.After successful modeling,the model group was divided into model control group,low dose group of Qufeng Mingmu Pill,and normal dose group of Qufeng Mingmu Pill,with 16 rats in each group.Rats in the low dose group and normal dose group of Qufeng Mingmu Pill were gavaged with Qufeng Mingmu Pill suspension 2g/Kg/ d and 4g/Kg/ d daily for 14 days,while the blank control group and model control group were gavaged with the same amount of distilled water daily.Detection of rno-mir-30b-5p and TLR4 m RNA expression in spleen and lymph node tissues by real-time fluorescence quantitative PCR;Detection of TLR4,My D88 and NF-κB p65 expression level in spleen and lymph node tissues by enzyme-linked immunosorbent assay(ELISA).Results: 1.The EAU rat model was successfully established.2.The levels of rno-mir-30b-5p and TLR4 m RNA were detected by q PCR,and the results showed that: The expression of rno-mir-30b-5p in the model control group was significantly lower than that in the blank group and the Qufeng Mingmu Pill intervention group(P<0.01),the difference between the low dose group and the normal dose group of Qufeng Mingmu Pill was statistically significant(P<0.05);the expression of TLR4 m RNA in the model control group was significantly higher than that in the blank group and the normal dose group of Qufeng Mingmu Pill(P<0.01),there was no significant difference between the low dose group and the high dose group of Qufeng Mingmu Pill(P>0.05).3.Detection of TLR4,My D88 and NF-κB p65 level in EAU rats by ELISA,the results showed that compared with the blank group,the expression of TLR4,My D88,NF-κB p65 in the model control group of protein was significantly higher than that of the blank group and the normal dose group of Qufeng Mingmu Pill(P<0.01),there was no significant difference in the expression of TLR4 and My D88 protein between the low dose and normal dose groups of Qufeng Mingmu Pill(P>0.05),the expression of NF-κB p65 protein between low dose and normal dose groups of Qufeng Mingmu Pill was significantly different(P<0.01).Conclusion: 1.The rno-mi R-30b-5p can negatively regulate the expression of TLR4 gene.2.TLR4/My D88/NF-κB conduction pathway is involved in the occurrence of autoimmune uveitis.3.Qufeng Mingmu Pill can inhibit TLR4/My D88/NF-κB conduction pathway can control the occurrence and development of autoimmune uveitis and play a therapeutic role.