| Object : To observe the effect of Qufeng Huoxue Pill on the expression of Bcl-2,Bax and Caspase-3 in experimental autoimmune uveitis rats retina,to explore the therapeutic effect and mechanism of Qufeng Huoxue Pill on EAU.Method : 12 of 65 healthy male Lewis rats(130 eyes)of SPF grade were randomly selected as blank group according to random digital table method.The remaining 53 rats were actively immunized to establish EAU rat model.After immunization,the ocular inflammatory reaction was observed under slit lamp microscope every day,and the inflammation score was evaluated according to the Caspi scoring standard.On the 7th day after the establishment of the model,5 rats were randomly selected from the model group and killed,and the eyeballs were taken to make pathological sections for histopathological examination.The successful EAU rats were randomly divided into three groups: model control group,Xiongdan Kaiming tablet group(positive drug control group),Qufeng Huoxue Pill low power group and Qufeng Huoxue Pill high power group.After 14 days of oral administration,the relative expression of Bcl-2 mRNA and Bax mRNA in retinal tissue was detected by qPCR method,and the relative expression of Caspase-3 protein in retinal tissue was detected by Western Blot method.Results:1.Establishment of EAU rat model: on the 4th day after active immunization in the model group,we observed that the iris vessels dilated,the blood vessels in the limbus corneum became hyperemia,and the red light reflex of some fundus weakened under slit lamp microscope.Ocular inflammation score(1.45 ±0.74).On the 7th day,the iris blood vessels were obviously dilated and hyperemia,the aqueous humor was turbid,the red light reflex of the fundus was obviously weakened,some of them were seen gray-white flocs on the pupil margin,the anterior chamber was suppurated,and the score of ocular inflammation was(2.62 ±0.65).The pathological sections of the eye of the rat showed different degree of inflammatory cell infiltration in the iris,the ciliary body and the retina after the cornea.The above shows that the EAU rat model was successfully established on Day 7 after active immunization.2.The results of qPCR showed that the expression of Bcl-2 mRNA in the model control group was lower than that in the blank group(P < 0.05),and the expression of Bcl-2 mRNA was significantly higher in the model control group(P <0.01).Compared with the model control group,the expression of Bcl-2 mRNA was significantly higher in Xiongdan Kaiming tablet group and Qufeng Huoxue Pill group(P < 0.01),but there was no significant change in Bcl-2 mRNA expression in Qufeng Huoxue Pill group(P > 0.05).The expression of Bax mRNA in Xiongdan Kaiming tablet group and Qufeng Huoxue pill high dose group was significantly lower than that in the model control group(P < 0.05),but there was no significant change in Bax mRNA expression in Qufeng Huoxue pill low dose group(P > 0.05).3.The results of Western Blot showed that compared with the blank group,the expression of Caspase-3in the model control group was significantly higher than that in the control group(P <0.01).Compared with the model control group,the expression of Caspase-3 in the low-fold group of Qufeng Huoxue Pill was lower than that in the model control group(P < 0.05),and the expression of Caspase-3 in the Xiongdan Kaiming tablet group and the high-level group of Qufeng Huoxue Pill was significantly lower(P < 0.01).Conclusion: 1.The EAU rat model was established by active immunization of Lewis rats with IRBP antigen-complete Freund’s adjuvant.2.Qufeng Huoxue Pill can up-regulate the expression of Bcl-2 and inhibit the expression of Bax,Caspase-3 in retinal tissue of EAU rats.These results suggest that Bcl-2,Bax and Caspase-3 may bethe targets of Qufeng Huoxue pills on EAU rats,thus improving the pathological process of EAU in order to play a therapeutic role.3.Qufeng Huoxue Pill may play an anti-inflammatory role by inhibiting the expression of apoptosis-related factor Bax,Caspase-3 and promoting the expression of Bcl-2 in uveitis. |
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