Observation Of The Effects Of Sangshu Yangzheng Decoction On Microecology And Immunity In Healthy Adults Based On Multi-omics Technolog

Purpose of the studySang Zhu Yang Zheng herbal tea(SZYZ),a government-recommended traditional Chinese medicine,was proven to be favourable in the prevention of respiratory tract infections(RTIs).However,its immunomodulatory effects and its effects on human microorganisms and metabolite profiles are not well known.The research methodsThis study was a randomized,blank control study.In this study,60 healthy volunteers were recruited.We determined the immunologic effect of SZYZ on healthy adults to understand the potential role of human microecology in enhancing the immunity of healthy adults.We also evaluated the impact of SZYZ on upper respiratory tract microorganisms and metabolism in the serum and feces of healthy adults using 16S rRNA sequencing and non-targeted metabonomic to gain insight into its mechanism of preventing respiratory tract infection.Research resultsAfter administration,the numbers of total T cells,total B cells,CD4+T cells,and CD8+T cells were profoundly increased.16S rRNA sequencing of the pharyngeal samples showed decreased diversity,enrichment of the genera Bacteroides and Bifidobacterium,and lowered abundance of the phyla Actinobacteria and Synergistetes and several opportunistic pathogens,including Actinomyces,Fretibacterium,Mobiluncus,and Cloacibacillus,after administration.The functions of microbes in the airway related to human disease pathways and bacterial infectious diseases were inhibited.No changes in diversity or composition were found in the gut microbiome after administration.Serum and fecal metabonomic analysis revealed changes in 138 and 197 metabolites with HDBM names,respectively.Of these,glycerolipids,glycerophospholipids,and sphingolipids were significantly elevated,whereas carboxylic acids,benzenes,fatty acyls,steroids,and steroid derivatives were down-regulated in serum.A KEGG pathway analysis predicted that tyrosine metabolism,biosynthesis of valine,leucine and isoleucine,and phenylalanine metabolism pathways were attenuated.Fecal metabonomics revealed that carboxylic acids,prenol lipids,organooxygen compounds,fatty acyls,phenol,indoles,coumarins,and flavonoids were significantly up-regulated.Based on a KEGG pathway analysis,pyrimidine metabolism,pantothenate and CoA biosynthesis,ubiquinone and other terpenoid-quinone biosynthesis,terpenoid backbone biosynthesis,and beta-alanine metabolism pathways may be involved.Analysis of the correlation among the airway microbe levels,lymphocyte count,levels of circulating metabolites,and levels of fecal metabolites revealed a network of the regulatory effects of SZYZ.Conclusion and significanceWe concluded that SZYZ enhanced lymphocyte counts,altered the microbial community in the airway and changed circulating and fecal metabolites in healthy humans,which provides possible insight into the immunomodulation and prevention of RTIs.