Study On The Mechanism Between Parahydroxycinnamaldehyde And Pulmonary Fibrosis Based On Animal Experiments And Network Pharmacology

Objective:Animal experiments and network pharmacology methods were used to explore whether p-hydroxycinnamaldehyde has the effect of treating pulmonary fibrosis and explore possible targets and mechanisms.Methods:The C57BL/6 mice were divided into blank control group,silic lung group,silic lung+ p-hydroxycinnamal group,and the lung coefficients,relative expression of IL-1β,TNF-α,COL-1 and FN-1 fibrous m RNA of each group were statistically analyzed,and the fibrosis changes of pathological sections after medication were observed,and the association between p-hydroxycinnamal and pulmonary fibrosis diseases was discussed,and then the chemical structure of p-hydroxycinnamal was obtained through the Pub Chem database by network pharmacological methods.The potential targets of p-hydroxycinnamal were predicted by Swiss Target Prediction database and Pharm Mapper database,and gene targets related to pulmonary fibrosis were screened with the help of Gene Cards database and Dis Ge Net database,and the potential targets of p-hydroxycinnamal acting on pulmonary fibrosis were obtained by mapping the p-hydroxycinnamal target with the disease target,and the common target was STRING 11.5 The platform constructs a protein interaction(PPI)network model,obtains PPI network and analyzes it.The common targets were introduced into the Metascape database for gene ontology(GO)functional enrichment analysis of potential targets of p-hydroxycinnamal against pulmonary fibrosis,and the Kobas database was used to carry out the enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway,the enrichment analysis results were visualized by R software 3.6.1,and the "compound-target-pathway" network diagram was drawn by Cytoscape 3.8.0.Results:Animal experiments showed that the relative expression of lung coefficients,IL-1β,TNF-α,COL-1 and FN-1m RNA increased in the silic lung group compared with the blank control group,and the degree of pulmonary fibrosis increased pathologically.Compared with the silicular group,the relative expression of lung coefficient,IL-1β,TNF-α,COL-1and FN-1m RNA decreased in the siliconiosis + p-hydroxycinnamal group,and the pathological section results showed that the degree of fibrosis was reduced after the administration of p-hydroxycinnamaldehyde,suggesting that p-hydroxycinnamal may have a certain alleviating effect on pulmonary fibrosis.Network pharmacology methods obtained 137 targets of p-hydroxycinnamaldehyde,5349 targets of pulmonary fibrosis,102 potential targets of p-hydroxycinnamal against pulmonary fibrosis,GO function enrichment analysis and KEGG pathway enrichment analysis found that p-hydroxycinnamal anti-pulmonary fibrosis mainly involved in 1015 biological processes,77 cell composition,126 molecular functions and 214 signaling pathways.。Conclusion:It is predicted that the effect of p-hydroxycinnamal on pulmonary fibrosis may be related to core targets such as ESR1,HSP90AA1,SRC,STAT3,AR,CCND1,HDAC1,MMP9,etc.,and its mechanism may be through the PI3K-Akt signaling pathway to regulate autophagy,differentiation and apoptosis of cells.