Based On The Network Pharmacology And In Vitro Experimental Study Trollius Chinensis Bunge Flavonoids The Mechanism Of Inhibition Of Pulmonary Fibrosis

Pulmonary fibrosis has become one of the main diseases of human death,seriously threatening human life and health.At present,the treatment of most pulmonary fibrotic diseases is extremely limited,and the drugs available are scarce.Globally,the leading drugs for the treatment of pulmonary fibrosis are pirfenidone and nintedanib,which have been clinically proven to be effective in inhibiting idiopathic pulmonary fibrosis,but the underlying gastrointestinal reactions,liver damage,bleeding and intolerance and other adverse effects,and the pathogenesis is unclear.Therefore,it is urgent to develop safe and effective pulmonary fibrosis treatment drugs.By using network pharmacology methods and establishing a TGF-β1-induced A549 cell pulmonary fibrosis model,this paper uses Trollius chinensis Bunge flavonoids,the main component extracted from nasturtium,as a feasible drug for the treatment of pulmonary fibrosis,to further improve the clinical efficacy and health needs,and provide preliminary experimental data and theoretical data.The specific work is as follows:(1)potential targets of pulmonary fibrosis were screened out by network pharmacological methods;With the help of traditional Chinese medicine system pharmacology database and analysis platform(TCMSP)to obtain drug active ingredient targets,while using Drug bank and Gene Cards database to obtain disease targets of pulmonary fibrosis,using Cytoscape software,using protein interaction,screening the core targets of nasturtin flavonoids for the treatment of pulmonary fibrosis,and using Metascape to carry out gene ontology on the core targets.GO)and enrichment analysis of the KEGG pathway.(2)In vitro experiments: the results of network pharmacology were verified by in vitro TGF-β1-induced A549 cell experiments;The gentium flavonoids were extracted and determined,and then the nasturtium flavonoids were divided into low,medium and high dose groups for intervention,on this basis,the scavenging capacity of superoxide anion radicals,hydroxyl radicals and DPPH was detected,and the oxidative stress related indexes such as superoxide dismutase(SOD),malondialdehyde(MDA),and reactive oxygen species(ROS)were measured to verify the antioxidant capacity of the drug.At the same time,qRT-PCR,Western blot,and immunofluorescence staining were used to detect the expression of EMT markers,and the effect of nasturtium flavonoids on pulmonary fibrosis was determined by the differences between groups.Combined with network pharmacological target prediction,potential Ak T-1 and p-Ak T-1 were detected,followed by the detection of the fibrotic classical signaling pathways p-Smad2/3,m TOR and NF-κB.This is used to derive the mechanism of nasturtium flavonoids acting on pulmonary fibrosis.