Studies On Chemical Constituents Of Polar Parts Of Pfaffia Glomerata And Its Antitumor Activity In Vitro

Pfaffia glomerata(Spreng)Pedersen,a herbaceous plant in the amaranth family,chemical constituents including steroids,triterpenes,flavonoids and alkaloids,which has anti-tumor,aphrodisiac and organ protective effects.In this study,chemical constituents of polar parts of P.glomerata were isolated by column chromatographic methods with D101 macroporous resin,silica gel,Sephadex LH-20,ODS,polyamide and preparative HPLC.The structures of obtained compounds were identified by UV,MS,NMR and physicochemical properties.Thirty three compounds were isolated from the ethyl acetate part of the 95%ethanol extract of P.glomerata,the methanol part of the 95%ethanol extract of P.glomerata and the n-butanol part of the 50%ethanol extract of P.glomerata.Thirty compounds named20 S,22S-O-(R-ethylidene)-20-hydroxyecdysone(1),20-hydroxyecdysone(2),polypodine B(3),poststerone(4),stachysterone B(5),pterosterone(6),calonysterone(7),taxisterone(8),makisterone C(9),20,22-O-(R-ethylidene)-20-hydroxyecdysone(10),20,22-O-(R-3-methoxycarbonyl)propylidene-20-hydroxyecdysone(11),shidasterone(12),oleanolic acid(13),oleanolic acid-28-O-β-D-glucopyranoside(14),5α-20-hydroxyecdysone(15),abutasterone(16),25-hydroxydacryhainansterone(17),chikusetsusaponin IVa methyl ester(18),1,2,4-benzenetriol(19),(-)-syringaresinol-4-O-β-D-glucopyranoside(20),syringaresinol(21),7,4＇-dihydroxy-5-methoxydihydroflavone(22),3-methyl-quercetin(23),luteolin(24),quercetin(25),N-transferuloyltyramine(26),methyl-α-D-frucofuranoside(27),methyl-β-D-frucofuranoside(28),sucrose(29),uridine(30)were identified.Among them,the compound 1 is a new compound.Compounds 1,3-5,7,9-12,15-17,19-20,27-28 and 30 were obtained from P.glomerata for the first time.Compounds 1,4-5,7,9-12,15-17,19-20,27-28 and 30 were isolated from the genus of Pfaffia for the first time.The isolated compounds(1-3,6-12,15-17)were screened for in vitro activity to inhibit the proliferation of cancer cells.The results findings that all compounds had inhibitory effects on the in vitro proliferation of A549 cells,MKN-45 cells,HEPG2.2.15 cells,HELA cells and MCF10 A cells.In addition,it was found that20 S,22S-O-(R-ethylidene)-20-hydroxyecdysone(1)inhibited the proliferation of MKN-45 cells and MCF10 A cells in vitro more than 20,22-O-(R-ethylidene)-20-hydroxyecdysone(10);20,22-O-(R-ethylidene)-20-hydroxyecdysone(10)inhibited the proliferation of HEPG2.2.15 cells in vitro more than 20 S,22S-O-(R-ethylidene)-20-hydroxyecdysone(1);20-hydroxyecdysone(2)inhibited the proliferation of MKN-45 cells in vitro more than 5α-20-hydroxyecdysone(15);5α-20-hydroxyecdysone(15)inhibited the proliferation of A549 cells,HEPG2.2.15 cells and MCF10 A cells in vitro more than 20-hydroxyecdysone(2).It was also found that the effect of inhibiting the proliferation of HELA cells in vitro was dose-dependent with steroids.