Expression And Clinical Significance Of HOXC10 In Lung Adenocarcinoma

Objective: To explore the expression of Homeobox gene C10(HOXC10)in lung adenocarcinoma(LUAD)and analyze its function and the potential mechanism,and complete correlation analysis based on clinical features.Preliminary discussion on the role of HOXC10 in LUAD and its clinical significance.Methods: The expression of HOXC10 in pan-cancer was evaluated in the TIMER database.Bioinformatics technology was used to further verify the expression of HOXC10 in LUAD data from the TCGA database and gene chip data GSE32863 and GSE43458 from the GEO database.The GSE68465 data set was grouped according to the median value of HOXC10 expression,divided into high and low expression groups,differentially expressed genes(DEGs)were screened,and the top 20 genes with the most significant up-regulation and down-regulation were selected to draw heat maps and the volcano maps of all DEGs.GO and KEGG enrichment analysis using cluster Profiler package in R,and then coexpression analysis of the top 20 most significantly up-and down-regulated genes.Construct protein-protein interaction network in STRING,and Cytoscape extracted key sub-networks.Prognostic analysis using KaplanMeier Plotter online database.Performed immune cell infiltration correlation analysis in TIMER and TISIDB databases.Immunohistochemistry was used to detect HOXC10 expression in 50 LUAD and 20 normal lung adjacent tissue specimens,and the relationship between HOXC10 expression and clinical features was analyzed.Finally,the receiver operating characteristic(ROC)curve was constructed to evaluate the diagnostic efficiency of HOXC10 for LUAD.Result: HOXC10 was highly expressed in LUAD.HOXC10-related DEGs were mainly enriched in leukocyte migration,cell chemotaxis,response to lipopolysaccharide,and other functions,and in the phagosomes,cytokine-cytokine receptor interaction the enrichment degree was high.CEACAM5,PCP4,TUBB2 B,and TFF3 were positively correlated with the HOXC10 expression,while CLEC4 A,MNDA,and P2RY13 were negatively correlated with HOXC10 expression.HOXC10 was most closely related to HOXC gene cluster members in the protein interaction network.Compared with the low expression group,the OS was significantly decreased in the high expression group of HOXC10.The expression of HOXC10 was negatively correlated with CD8+T cells.The positive expression rate of HOXC10 was higher than that of normal adjacent lung tissues(P<0.05),and was correlated with smoking,TNM stage,lymph node metastasis,and distant metastasis(P<0.05),but not with age,sex,or tumor size.The area under the ROC curve(AUC)was0.680(P<0.05).Conclusion: HOXC10 was highly expressed in LUAD and correlated with smoking,TNM stage,lymph node metastasis,and distant metastasis.Bioinformatics showed that HOXC10 may regulate the development of LUAD through leukocyte migration,cell chemotaxis,and response to lipopolysaccharide,as well as phagosome and cytokine-cytokine receptor interactions.HOXC10 can be used as a potential molecular marker to aid in the prognosis assessment of LUAD.