| Objective:To analyze the relationship between five single nucleotide polymorphism loci of NOS3 gene and susceptibility to diabetic kidney disease in patients with type 2diabetes,and to provide certain basis for prevention and treatment of clinical type 2diabetic kidney disease.Methods:A total of 241 subjects were included,including 90 patients with type 2 diabetes mellitus,70 patients with type 2 diabetic kidney disease,and 81 healthy control subjects from the medical checkup center population,all of whom were randomly selected from patients hospitalized in China-japan Union hospital of Jilin University and the healthy population of the medical checkup center between 2015 and 2017,and the three groups were collected for gender,age,body mass index(BMI),systolic blood pressure,diastolic blood pressure.And hematological indexes were collected from patients,including fasting blood glucose(FBG),glycated haemoglobin(HbA1c),blood urea nitrogen(BUN),serum creatinine(Scr),cystatin C(Cys C),serumuric acid(SUA),triglycerides(TG),total cholesterol(TC),cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),apolipoprotein A1(ApoA1),apolipoprotein B(ApoB),lipoprotein(a)(LP(a)),blood magnesium,lymphocyte(LY)and platelet count(PLT).The genotypes and alleles of each SNP locus were used to study their relationship with the development of type 2diabetic kidney disease.The data were statistically analyzed by SPSS26.0 statistical software.Results:1.The age and the proportion of male patients in the T2 DM and DKD groups were significantly higher than those in the healthy control group,with statistical significance(P < 0.05).Urea nitrogen,creatinine,cystatin C,systolic blood pressure and diastolic blood pressure in the DKD group were significantly higher than those in the T2 DM group,with statistically significant differences(P < 0.05).2.The frequency of AG genotype at the rs1800779(A/G)locus of NOS3 gene was higher in the DKD group than in the healthy control group,and the difference was statistically significant(P < 0.0167).3.The frequency of the G allele at the rs1800779 locus of the NOS3 gene was higher in the DKD group than in the T2 DM group,and the difference was statistically significant(P < 0.0167).4.Rs11771443,rs1799983,rs3918227 and rs7830 loci of NOS3 gene did not show any statistical difference in genotype distribution frequency and allele distribution frequency among the three groups(P > 0.05).5.Rs11771443 locus TT genotype group had higher total cholesterol than CC genotype and TC genotype group,and rs1800779 locus AA genotype group had higher BMI and systolic blood pressure than AG genotype group,and the differences were statistically significant(P < 0.05).6.Logistic regression analysis of risk factors associated with DKD yielded cystatin C(OR=5.116,95% CI 1.17-22.36,P=0.03)and rs1800779 locus AG genotype(OR=2.857,95%CI 1.43-5.702,P=0.003)as independent risk factors for DKD(P<0.05).Conclusions:1.Elevated cystatin C and the AG genotype at the rs1800779 locus were independent risk factors for DKD,and the risk of diabetic kidney disease increased by 41% for each unit increase in cystatin C.The risk of diabetic nephropathy in the population with the AG genotype at the rs1800779 locus was 2.79 times higher than that in the non-AG genotype population.2.The polymorphism of NOS3 gene rs1800779 locus was correlated with the development of type 2 diabetic kidney disease in Changchun population,and the genotype AG as well as allele G of this locus increased the risk of developing DKD.3.Rs11771443 locus of NOS3 gene may be associated with increased total cholesterol level in DKD patients;rs1800779 locus may be associated with increased BMI and systolic blood pressure in DKD patients. |
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