Study On The Relationship Between Angiotensin â…  Converting Enzyme Gene Polymorphism And Diabetic Kidney Disease In Patients With Type2Diabetes Mellitus

OBJECTIVE This experiment with PCR method to detect ACE gene I/D polymorphism and Serum ace level.To investigate whether the insertion/deletion polymorphism of Angiotensin I converting enzyme (ACE) gene and serum ACE levels are associated with diabetic kidney disease in patients with type2diabetes mellitus. Thus for T2DM-DKD+patients early clinical using ACEI drugs treatments supply with the theory reference and experimental basis.METHODS ACE gene types were analyzed in63controls.69cases with T2DM-DKD+,75cases with T2DM-DKD-by polymerase chain reaction(PCR). ACE levels were measured with enzyme-linked immunoassay(double antibody sandwich).RESULTS1The DD gene type frequency and D allele frequency were higher in T2DM-DKD+as compared with T2DM-DKD-and controls (P<0.05). The odds ratio associated with DD/â…¡ gene type was3.19(P<0.05) for T2DM-DKD+2Patients with T2DM-DKD+had higher serum ACE levels compared with T2DM-DKD-and controls (P<0.05)CONCLUSION Deletion polymorphism of the ACE gene might be an important risk factor in type2diabetes mellitus patients with diabetic kidney disease...