Study On The Correlation Of Metabolic Syndrome With Sex Hormone Binding Protein And Testosterone In Obese Boys

Objective:To explore the correlation of sex hormone-binding globulin(SHBG)and Total Testosterone(TT)with the development of Metabolic syndrome(MetS)in obese boys.Methods:A total of 439 boys aged 6-18 years old from April 2020 to February 2023,include boys who visited the Department of Endocrinology,Genetics and Metabolism in Jiangxi Province Children’s Hospital and healthy boys who underwent physical examination in our hospital were collected.According to the diagnostic guidelines for metabolic syndrome,they were divided into Control,CON group(76);Simple obesity(OB)group(249);Metabolic syndrome(MetS)group 114 people.General information,physical measurement and physical examination data,laboratory test results and peripheral blood serum were collected.spss26.0 software was used to analyze and compare the differences of metabolic indices between MetS group,CON group and OB group.The relationship of TT and SHBG level among components was analyzed.Eight boys randomly from each three group were selected as subjects for targeted proteomic analysis,screening differential protein analysis,functional analysis of differential protein analysis and protein interaction network analysis.Result:1.Clinical data analysis:The CON group,OB group and MetS group were pairwise compared,and age,puberty and Hb A1 c were no statistically significant differences(P > 0.05)in three pairwise comparisons,respectively.Compared with CON and OB groups,body mass index(BMI),systolic blood pressure(SBP),diastolic blood pressure(DBP),Low density lipoprotein(LDL-C),non-high-density lipoprotein cholesterol(non-HDL-C),triglyceride(TG),total cholesterol(TC),Fasting blood glucose(FBG),Fasting insulin(FINS),insulin resistance index(HOMA-IR),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were higher in MetS group,and high density lipoprotein cholesterol(HDL-C),Free fatty acid(FAA),SHBG and TT levels were lower,and the differences were statistically significant(P< 0.05).The correlation between TT and other variables in the sample was analyzed,and the results showed that TT level was independently correlated with puberty,TC and age,with statistical significance(P<0.05).The correlation between SHBG and TT and other variables in the sample was analyzed,and the corresponding independent correlation factors were screened out.After correcting confounding factors,the prediction of SHBG and TT on the risk of metabolic syndrome was analyzed.The results showed that the changes of SHBG and TT in CON group and OB group were statistically significant compared with MetS group(P< 0.05),and the OR and 95%CI of SHBG were 2.308(1.678-3.175)and 1.271(1.199-1.347),respectively.The OR and 95% CI of TT were 1.012(1.005-1.019)and 1.009(1.003-1.015),respectively.2.Proteomic analysis:(1)Screening differential protein analysis: The CON and MetS groups,the CON and OB groups,and the OB and MetS groups all showed significantly down-regulated differential proteins.(2)Protein interaction network analysis: It can be seen that the proteins associated with SHBG are IGF1,IGFBP1 and CRP.Conclusions:1.Low TT can be an independent risk factor for the development of metabolic syndrome in boys.2.Decreased SHBG level is an independent risk factor for MetS in boys.3.BMI,SBP,DBP,LDL-C,non-HDL-C,TG,TC,FBG,FINS,HOMA-IR,ALT and AST of MetS boys were higher than those of healthy boys and obese boys;HDLC,FAA,SHBG and TT were lower than healthy boys and obese boys.