Clinical Correlation Between Blood Biomarkers And Parkinson’s Disease

Purpose:To investigate the relationship between the Parkinson’s disease and control groups for homocysteine(Hcy),neuron specific enolase(NSE),total cholesterol(TC),apolipoprotein A1(Apo-A1),apolipoprotein B(Apo-B),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),neutrophil,neutrophil to high-density lipoprotein ratio(NHR),monocyte,monocyte to high-density lipoprotein ratio(MHR),lymphocyte(LYM),neutrophil to lymphocyte ratio(NLR),platelets,platelet lymphocyte ratio(PLR),total bilirubin,hemoglobin(Hb),glutathione reductase(GR),and uric acid,to investigate the independent risk factors and protective factors of blood biomarkers in patients with Parkinson’s disease(PD)and The correlation analysis of the above indicators with the severity of PD.Methods:Retrospective collection of 166 patients diagnosed with Parkinson’s disease who were hospitalized between October 2020 and March 2023 at the First Affiliated Hospital of Nanchang University and 171 patients with non-Parkinson’s disease who were hospitalized during the same period were collected as a control group.The PD group was divided into early PD group and late PD group according to Hoehn and Yahr(H&Y)staging.The personal data and experimental data of all included patients were collected for statistical analysis.The categorical variable of gender was analyzed by?2test or Pearson’s exact test;the continuous variables that conformed to the normal distribution were analyzed by independent sample t test,expressed as mean±standard deviation;Continuous variables with normal distribution were analyzed by Mann-Whitney U test,expressed as median(quartile);due to the collinearity of TC,NHR,NLR,MHR,PLR and other indicators above,TC will not be included statistical analysis,considering that NHR,NLR,MHR,PLR and PD have research significance with each other,they were separately analyzed by Mann-Whitney U test;The statistically significant indicators obtained from the above statistical analysis will be subjected to multivariate logistic regression analysis to evaluate their association with PD,and the receiver operating characteristic curve(ROC)will be drawn to evaluate the ability of LYM,HDL-C,and Hcy to predict the occurrence of PD in patients;Spearman correlation analysis was performed on the above blood indicators,gender,age and PD severity and a box plot was drawn.Results:1.The LYM of the PD group was(1.51±0.47)*10^9/L,while that of the control group was(1.65±0.51)*10^9/L.The LYM of PD patients was lower than that of the control group,and the difference was statistically significant(P=0.01);The NLR of the PD group was 2.24(1.64–2.96),while the NLR of the control group was 1.95(1.43–2.50).The NLR of the PD group was higher than that of the control group,and the difference was statistically significant(P=0.036);The Hcy of the PD group was12.05(10.31–14.57)μmol/L,while that of the control group was 10.20(8.70–12.46)μmol/L.The Hcy of the PD group was significantly higher than that of the control group,and the difference was statistically significant(P<0.001);The remaining indicators showed no statistically significant difference between the PD group and the control group(P>0.05).2.Multivariate logistic regression analysis found that there were still statistical differences in LYM,Hcy,and HDL-C.The results indicate that a decrease in LYM and an increase in Hcy and HDL-C may be independent risk factors for PD.Every increase of 1 unit in Hcy leads to a 21.2%increase in the risk of developing PD in patients;For every 1 unit increase in HDL-C,the risk of PD patients increases by 260.7%.3.The ROC curve predicts the risk of PD for LYM,Hcy,and HDL-C,with an area above the LYM curve of 0.421(95%CI,0.355–0.486)and an area below the Hcy curve of 0.669(95%CI,0.609–0.730),which is higher than the area under the HDL-C curve of 0.585(95%CI,0.520–0.650).4.The age of late stage PD patients was higher than that of early stage PD patients(P<0.05);The Apo-B and LDL-C levels in late stage PD patients were lower than those in early stage PD patients(P<0.05);There was no significant difference in gender and other blood biomarkers between early and late PD.5.The Spearman correlation coefficient r_s between age and the severity of PD was0.264(P<0.01),and age was positively correlated with the severity of PD;the r_s of LDL-C,Apo-B and the severity of PD were respectively-0.160,-0.196(P<0.05);LDL-C and Apo-B were negatively correlated with the severity of PD.Conclusion:1.LYM may be a protective factor for PD;Hcy and HDL-C may be independent risk factors for PD;2.LDL-C and Apo-B were negatively correlated with the severity of PD,while age was positively correlated with the severity of PD.3.The NLR of the PD group is higher than that of the control group,and NLR may be a risk factor for PD.