The Correlation Analysis Of Peripheral Blood Immune Related Cells And Cytokines With Parkinson’s Disease

Objective:To investigate the changes in peripheral blood immune-related cells and cytokines in patients with the Parkinson’s disease(PD),and to evaluate their association with the severity of PD.Methods:In this study,60 PD patients and 50 healthy people were recruited from the Department of Neurology of Jiangxi Provincial People’s Hospital from July 2019 to December 2020.The lymphocytes and cytokines in peripheral blood were analyzed by flow cytometry.UPDRSIII and H-Y chart were used to assess motor symptoms and its severity,respectively.MMSE and MOCA scores were used to evaluate cognitive function.Moreover,the anxiety and depression were evaluated by HAMILTON.The levels of peripheral blood lymphocytes and cytokines in two groups were compared,and their correlation with the scores of UPRDSIII,H-Y,MMSE,MOCA and Hamilton Anxiety and Depression Scale in PD patients was analyzed.Results:1.The number of T lymphocytes,CD4+T lymphocytes and Treg lymphocytes in the PD group were lower than those in the control group,while the number of NK lymphocytes was higher than that in control group(P < 0.05).The number of lymphocytes and B lymphocytes in the PD group were lower than those in the control group,while the number of CD8+T lymphocytes was higher than that in control group(P > 0.05).The percentage of T lymphocytes,CD4+T lymphocytes and CD4+T/CD8+T in PD group were lower than those in control group,while the percentage of NK cell was higher than that in control group(P<0.05).The percentage of B lymphocytes and Treg lymphocytes in the PD group were lower than those in the control group(P>0.05).2.The percentage of CD4+T lymphocytes and CD4+T/CD8+T in PD group had a negative correlation with UPDRSIII scores,while the percentage of NK lymphocytes had a positive correlation with UPDRSIII scores(P<0.05).The percentage of CD4+T lymphocytes and CD4+T/CD8+T in PD group had a negative correlation with H-Y stagel,while the percentage of CD8+T and NK lymphocytes had a positive correlation with H-Y stagel(P < 0.05).The total number of T lymphocytes had a positive correlation with Moca scores,while the NK lymphocytes had a negative correlation with Moca scores(P<0.05).No significant correlation was found between Treg and B cells and the severity of the disease.3.We assessed flow cytometric data of PD patients divided into 3 groups based on UPDRS-III scores:PD-1,PD-2 and PD-3 group.The percentage of CD4+T and CD4+T/CD8+T cells in PD-1 group were higher than those in PD-2 group and PD-3group,respectively.The pairwise comparison showed that there were significant differences between PD-1 and PD-3,and between PD-2 and PD-3.The percentage of CD8+T cells in PD-1 group were lower than those in PD-2 group and PD-3 group,respectively.The pairwise comparison showed that there were significant differences between PD-1 and PD-3,and between PD-2 and PD-3.We also assessed flow cytometric data of PD patients segregated into 3 groups based on H-Y stagel:PD-I,PD-II and PD-III group.The percentage of T cells,CD4+T cells and CD4+T/CD8+T in PD-I group were higher than those in PD-II group and PD-III group,respectively.The pairwise comparison showed the significant differences between PD-I and PD-II,and between PD-I and PD-III group.The percentage of NK cells were lower than those in PD-II group and PD-III group,respectively.The pairwise comparison showed the significant differences between PD-I and PD-II,and between PD-I and PD-III group.Then,we divided PD patients into 2 groups based on Moca scores:PD-D and PD-ND.The percentage of T cells and CD4+T cells in the PD-D group were lower than those in the PD-ND group,while the NK cells in the PD-D group were higher than those in the PD-ND group.According to the anxiety and depression HAMILTON scale,the patients with PD were divided into 3 groups,and there was no significant difference among the three groups.4.The level of INF-γ,INF-γ/IL-4 and IL-17/Treg in PD group were higher than those in control group,while IL-4,IL-17 and IL-10 in PD group were decreased in control group(P>0.05).The level of IL-6 and IL-1β in PD group were higher than those in control group(P<0.05).Conclusion:1.The changes in the peripheral blood immune cells and pro-inflammatory cytokines in PD patients may suggest the involvement of immune inflammation in the pathogenesis of PD.2.The percentage of total T cells,CD4+T cells,NK cell,and CD4+T/CD8+T has a correlation with the severity of PD,which will provide a promising strategy for diagnosis and treatment of PD,and evaluating the progression of PD.