Analyzing The Distribution Patterns Of Plasmacytoid Dendritic Cells In Ulcerativelesions Of Digestive Tract

Research background and purposePeptic ulcer is one of the most common benign digestive system diseases in the world.Combined with the literature,our research group found that in many cases of digestive tract ulcer diseases are often accompanied by plasmacytoid dendritic cells(pDCs)proliferation.This phenomenon does not exist in general chronic inflammation of the digestive tract.So what is the clinical significance of pDCs proliferation in ulcerative lesions?Are they involved in autoimmune response?Can they affect the outcome of the disease?The study on the distribution pattern and distribution rule of pDCs in digestive tract ulcers is helpful to distinguish malignant tumors,especially lymphoid hematopoietic tumors,provide direction for follow-up basic research,and analyze the development mechanism of the disease.Research materials and methods328 patients with suspected pDCs hyperplasia were collected from Nanfang Hospital from 2014 to 2019 and Huayin pathological diagnosis Center in 2019,including 222 male patients and 106 female patients.Paraffin blocks were used for section.Through the microscopic observation of HE section and immunohistochemical evaluation,to explore the distribution patterns of pDCs marked by CD 123 and to evaluate the distribution of pDCs.IBM SPSS Statistics 20 software and RStuido software were used for statistical analysis,Chi-square test,Fisher accurate test and Binary Logistic regression were used for qualitative evaluation.One-Way ANOVA,Mann-Whitney U test and Kruskal-Wallis H test were used to compare the total geometric mean or average rank among groups.Tamhane’s T2 test,Mann-Whitney U test or t test of two independent samples were used to compare the two groups.In the part of clinicopathological data,Pearson linear correlation analysis and Linear regression analysis were used to construct the linear regression equation.Research resultsAmong the non-specific ulcers of the digestive tract,53.7%of the cases were accompanied by the distribution of pDCs.Statistical analysis showed that there was no significant difference in the distribution rate of pDCs between benign and malignant digestive tract ulcerative lesions and inflammatory bowel diseases.However,the distribution of pDCs was the most in the esophagus.Except for IBD,the distribution of pDCs in large intestine was higher than that in small intestine,and the distribution of pDCs in Crohn’s disease was higher than that in ulcerative colitis.PDCs could also be found in disease such as inflammatory bowel diseases,lymphadenopathies,IgG4-related diseases,chronic nasopharyngitis,gastrointestinal ulcers caused by specific infection and gastrointestinal ulcers caused by radiotherapy and chemotherapy,but the distribution were different.Main conclusion1.More than half of digestive tract ulcers were accompanied by the distribution of pDCs,and pDCs were involved in the occurrence of ulcer.2.pDCs were clustered or scattered,often appeared in the background of ulcer,and could be found in mucosal erosion area,lymphoid tissue proliferation area or squamous epithelium.3.pDCs were more common in the middle or bottom of the ulcer,and the distribution of the ulcer was less when the ulcer was superficial.4.The distribution of pDCs in esophagus was the most.Furthermore,except for IBD,the distribution of pDCs in large intestine was higher than that in small intestine.Moreover,the distribution of pDCs in Crohn’s disease was higher than that in ulcerative colitis.5.There was a positive correlation between the distribution of pDCs and the number of ulcer’s visual field under microscope.6.The distribution of pDCs could be seen in the control studies such as lymphadenopathies,tonsillar tissue,Crohn’s disease without ulcers and IgG4-related diseases,suggesting that pDCs were involved in immune response.7.The distribution of pDCs in malignant ulcers of digestive tract suggests that pDCs was involved in the development of tumor,while in lymph node metastasis,pDCs were not found in carcinomatous stroma,suggesting that pDCs might be related to the inhibition of tumor metastasis.