Study On Chemical Constituents Of Patrinia Villosa

Objective: To study the chemical constituents of Patrinia villosa Juss and search the pilot of parent compounds with novel structure and better biological activity.Methods: Total of dried Patrinia villosa Juss was extracted by percolation with 95%ethanol,the constituents were isolated and purified by column chromatography,and identified by physicochemical properties and spectroscopic method.Results: 32 compounds were isolated and identified from Patrinia villosa Juss,as follows: patriviside A(1),patriviside B(2),patriviside C(3),(4β,8β)-8-ethoxy-3-methyl-10-methylen-2,9-dioxatricyclo[4.3.1.03,7]decan-4-ol(4),8,9-didehydro-7-hydroxydolichodial(5),(3S,4R,5S,8S,9S)-3,8-epoxy-7-hydroxy-4,8-dimethylperhydrocyclopenta[c]pyran(6)1′,3′-dioxane[6,7]cyclopenta[c]pyran-3(1H)-one,hexahydro-2′,2′,4-trimethyl-,(4S,4a S,6R,7R,7a S)(7)patrinoside(8),villosol(9),rupesin E(10),ent-edudesm-4(15)-1β,6α-diol(11),(S)-3-hydroxy-β-ionone(12),5,3’,4’-trihydroxy-3,7-dimethoxyflavone(13),rutin(14),5,7,4’-trimethoxyflavone(15),3,5,7,4’-tetrahydroxyflavone(16),3,5,3’-trimethoxyquercetin(17),rhamnocitrin(18),pinoresinol(19),(1S,3a R,4S,9a R)-1-ethoxy-4-4′-hydroxy-3′-methoxyphenyl)-7-methoxy-1,3,3a,4,9,9a-hexahydronap htho[2,3-c]-furan-6-ol(20),(+)-isolariciresinol(21),scopoletin(22),ursolic acid(23),ursulate lactone(24),stigmasterol(25),spinasterol(26),2’,3-dihydroxy-5-methoxydibenzyl(27),ferulic acid(28),P-hydroxybenzoic acid(29),P-ethoxybenzyl alcohol(30),trans-P-coumaric acid ethylester(31),2,5-dihydroxyphenylethyl acetate(32).compounds 1 and villosol were screened for cytotoxic activity,and the results showed that the new compound 1 had no significant activity,and villosol had a good inhibitory effect on human cervical cancer cells.Conclusion: 32 compounds were isolated from Patrinia villosa Juss.Among them,compounds 1-3 were new compounds.Except compounds 5,9,14,23,25 and 28,the other compounds were isolated from Patrinia villosa Juss for the first time.The new compound 1 has no significant activity,and villosol has a good inhibitory effect on human cervical cancer cells.