| Objective Pingchuan Ⅰ is Professor Yang effective treatment of acute asthma attack prescription.In this study,RSV asthma mouse model was established to observe the effect of Pingchuan Ⅰ on the regulation of RORγt and Foxp3 mRNA in the lung tissue of asthmatic mice at different periods to explore the mechanism of action of Pingchuan Ⅰ in the treatment of asthma.Methods Ninety healthy male BALB / c mice were randomly divided into three batches,which were challenged and sacrificed at2,4 and 6 weeks respectively.Each batch was divided into normal control group,asthma model group,dexamethasone group,Pingchuan Ⅰ low,medium and high dose group,5 in each group.Faure marin inactivated respiratory syncytial virus(FI-RSV)was sensitized and stimulated.Mice were sensitized twice on the first day and the eighth day,respectively.Sensitive when the FI-RSV and aluminum hydroxide 1: 1configuration,sensitized mice by intraperitoneal injection,the normal control group was replaced with an equal amount of saline instead of sensitization;two hours after the second sensitization,according to the group respectively dexamethasone and Pingchuan Ⅰ low,medium and high doses of intragastric administration of mice in each treatment group,the normal control group and asthma model group with an equal volume of distilled water instead of gavage,continuous gavage until the end of each batch.After the pentobarbital sodium anesthesia with FI-RSV nasal drip to stimulate the acute asthma attack in mice model of asthma,each batch of mice were killed 24 hours after the last challenge,conventional left middle of the right lung,using the Soviet Union Pathomorphology observation was made by HE staining.The remaining lung tissues were detected by quantitative real-time polymerase chain reaction(q RT-PCR)RORγt and Foxp3 mRNA expression levels.Results The pathological sections of lung tissue showed that the normal control group had mild pathological damage.The asthma model group observed a large number of inflammatory cell infiltration and obvious pathological damage,and its severity: the third group was the first batch of the second batch.Inflammation and pathological damage degree in each treatment group were lighter than asthma model group,and is inversely proportional to the duration,the degree of Pingchuan Ⅰ low,middle dose group are similar,dexamethasone group and Pingchuan Ⅰ high dose group are similar,and lower than the Pingchuan Ⅰ low,middle dose group.The expression level of transcription factors,in each batch of each group,asthma model group ROR gamma t mRNA expression levels were significantly higher than normal control group(P < 0.01),Foxp3 mRNA the expression level of the normal control group decreased significantly(P < 0.01);ROR gamma tmRNA expression level of the treatment group were lower than the asthma model group(P < 0.05),the expression of Foxp3 mRNA levels were higher than in asthma model group(P < 0.05),Pingchuan Ⅰ low and middle dose group are similar(P﹥0.05),dexamethasone group and Pingchuan Ⅰ high dose group are similar(P﹥0.05),and the expression level of the ROR gamma tmRNA in Pingchuan Ⅰ high dose group and dexamethasone group are less than Pingchuan Ⅰ low and middle dose group(P< 0.05),and the expression level of the Foxp3 mRNA are higher than Pingchuan Ⅰ low and middle dose group(P < 0.05).Among the three groups,the first batch and the third batch of the asthmatic model group,the dexamethasone group and Pingchuan Ⅰ high dose group were different(P<0.05).Conclusion The imbalance between RORγt and Foxp3 mRNA is involved in the airway inflammatory processes of asthma.Both Pingchuan Ⅰ and dexamethasone can down-regulate the expression of RORγtmRNA and up-regulate the expression of Foxp3 mRNA,and the high dose of Pingchuan Ⅰ is similar to dexamethasone... |
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