Experimental Study On The Effect Of Self-made Zicao Oil For External Use On Allergic Contact Dermatitis In Mice

Research purposes: In this experiment,self-made Zicao oil is used to treat allergic contact dermatitis in mice,and then to explore and discuss the efficacy and possible mechanism of self-made Zicao oil in the treatment of allergic contact dermatitis.Theself-made Zicao oil provides a theoretical basis for the treatment of children’s allergic contact dermatitis.Experimental method: 70 male SPF(fluocinolone acetate cream group),olive oil group,selfmade Zicao oil low concentration group(1/2 times concentration),self-made Zicao oil group,selfmade Zicao oil high concentration group(2 times the concentration).2,4-Dinitrofluorobenzene(DNFB)was used to induce the establishment of a mouse model of allergic contact dermatitis(ACD).The changes of mouse ear swelling degree and serum IL-1β,IL-6,TNF-α protein content changes after drug intervention were observed after the application of smear,and the efficacy and mechanism ofself-made Zicao oil in the treatment of children’s ACD were explored.Experimental results: The ear swelling of ACD mice was significantly inhibited by self-made Zicao oil group,high concentration group of self-made Zicao oil and positive control group(P <0.01).The ear swelling of ACD mice was significantly inhibited by low concentration group of selfmade Zicao oil(P < 0.05),but the inhibitory effect of olive oil group was not obvious,and there was no statistical difference(P > 0.05).External application of self-made Zicao oil,high concentration group of self-made Zicao oil and positive control group could significantly reduce the serum protein level of model mice(P < 0.01).Low concentration group of self-made Zicao oil could significantly reduce the serum protein level of model mice(P < 0.05),while olive oil group could reduce the serum IL-1β,IL-6 and TNF-α protein content,which was not considered statistically significant(P >0.05).Research conclusion: Experiments on the effect of self-made Zicao oil on mouse ACD model show that self-made Zicao oil has therapeutic effect on mouse ACD model,IL-1β,IL-6,and TNF-α.