Dendrobium Huoshanense Improves Doxorubicin-induced Heart Failure In C57BL/6 Mice

Heart failure refers to a group of complex clinical syndromes caused by various heart structures or functions that lead to ventricular filling or reduced ejection capacity,which can lead to dyspnea,fatigue,fluid retention and other clinical manifestations.Heart failure has high morbidity and mortality,which often causes respiratory tract infection,electrolyte disorder,cardiogenic cirrhosis and other complications.And the recurrence of the disease seriously threatens human life and health.Considering factors such as poor tolerance of western medicines,it has become a new research direction to find a safe and less toxic and side effects therapy.Dendrobium huoshanense(DH)is a kind of nourishing herb and health food,which has the functions of enhancing immunity,anti-tumor,inhibiting platelet coagulation,anti-oxidation and anti-aging.However,the effects of DH in heart failure are still unclear.In this study,we used doxorubicin-induced chronic heart failure mouse model to explore the therapeutic effect and mechanism of DH on heart failure.We randomly divided eight-week-old male C57BL/6 mice into three groups: namely the control group(Ctrl),the doxorubicin group(DOX)and the Dendrobium huoshanense treatment group(DH).Chronic heart failure was induced by intraperitoneal injection of doxorubicin solution.Mice in DH group were fed normal chow containing 1.5% DH powder,and the Ctrl and DOX groups were fed normal food.After 4-weeks treatment,electrocardiograms were measured.The mice were sacrificed,serum and heart samples were collected,and biomarkers related to mouse heart injury were detected.The heart tissues were conducted HE,Masson,Sirius red staining and TUNEL staining to determine cardiac tissue morphology,fibrosis,collagen content and apoptosis,respectively.m RNA and protein expression were determined by q RT-PCR and Western blot,respectively.Our results show that DH reduced the DOX-induced serum biomarkers creatine kinase(CK),lactate dehydrogenase(LDH)and aspartate aminotransferase(AST)of heart damage and reduced heart fibrosis.Mechanically,DH inhibited myocardialapoptosis,decreased interleukin 6 and tumor necrosis factor α levels,but increased superoxide dismutase 2 expression.Our research also suggest that DH is a promising Chinese herbal medicine for treatment of heart failure,and laid a foundation for the clinical treatment of heart failure.