Correlation Between Neuregulin-4 Levels And Endothelial Function In Primary Elderly Hypertension Patients

Objective:To explore the serum level of Nrg4 and its correlation with endothelial function in elderly patients with primary hypertension.Methods:In this study,84 patients with primary elderly hypertension who we re hospitalized in the Department of Cardiovascular Medicine of the Sec ond Hospital of Hebei Medical University from February 2020 to Dece mber 2020 were randomly selected as the test group,including 44 male s and 40 females,with an average age of(73.20±5.48)years,and the h ypertension was divided into 3 subgroups(28 patients with hypertension grade 1,14 males and 14 females,27 patients with grade 2 hypertensi on,16 males and 11 females,29 cases of hypertension grade 3,14 mal es and 15 females.Forty five patients with normal physical examination were selected as the control group,including 21 males and 24 females,with an average age of(72.87±6.19)years.Serum levels of neuregulin-4(Nrg4),endothelin-1(ET-1)and von Willebrand Factor(v WF)were dete rmined by enzyme linked immunosorbent assay.Serum levels of nitric o xide(NO)were assayed by nitric acid reduction.SPSS23.0 was used fo r statistical data processing and analysis.The measurement data were d escribed by means±standard deviation((?)±s),and the count data we re described by percentage.The t test was used to compare the measure ment data.For intra-group data comparison,analysis of variance was u sed for homogeneity of variances,and nonparametric test was used for heterogeneity.The enumeration data were analyzed by x~2.Pearson correl ation analysis was used to analyze the correlation between serum NO,E T-1,v WF and serum Nrg4.and P<0.05 indicated statistically significant difference.Results:1.Compared with the control group,the serum Nrg4 level in the h ypertension group was significantly decreased(58.24±16.56pg/ml vs87.80±12.43pg/ml),and the difference was statistically significant(P<0.01).T he pairwise comparison within the hypertension group had statistical sig nificance.(76.03±4.56pg/ml vs 58.45±9.15pg/ml vs 40.86±9.26pg/ml,P<0.01).2.Serum NO level in the hypertension group was significantly lower than that in the control group(13.81±2.87umol/L vs 32.53±6.08ummol/L),and the difference was statistically significant(P<0.01).There was statistical significance in pair comparison of hypertension group(16.04±1.74umol/L vs14.56±1.70ummol/L vs 10.96±2.20ummol/L,P<0.01).3.Compared with the control group,serum ET-1 level in the hypertension group was significantly increased(75.07±18.15ng/L vs25.73±5.41ng/L),and the difference was statistically significant(P<0.01).Pair comparison within the hypertension group was statistically significant(61.03±10.43ng/L vs 70.03±10.54ng/L vs 93.32±14.21ng/L,P<0.01).4.Compared with the control group,the serum v WF level in the hypertension group was significantly increased(89.11±19.05U/L vs52.48±8.99U/L),and the difference was statistically significant(P<0.01).Pair comparison of hypertension group showed statistical difference(74.72±9.38U/L vs 83.06±10.51U/Lvs 108.64±15.85U/L,P<0.01).5.According to Pearson correlation analysis,Nrg4 level was positively correlated with NO level(r=0.80,P<0.01)and negatively correlated with ET-1(r=-0.80,P<0.01),v WF level(r=-0.79,P<0.01)and BMI(r=-0.31,P<0.01)in the hypertension group.In the control group,there was no significant correlation between serum Nrg4 and all indexes.6.According to Pearson correlation analysis,there was insignificant correlation between age and NO level(P=0.635),ET-1(P=0.608)and v WF(P=0.707)in the hypertension group.7.Multiple linear regression analysis was conducted with NO,ET-1 and v WF as dependent variables and age,BMI,TC,TG,LDL-C and HDL-C as independent variables in the hypertension group.The results showed that Nrg4was the influencing factor of v WF(β=-0.729,P<0.01).Conclusions:1.The decrease of serum Nrg4 level in primary elderly patients with hypertension is correlated with vascular endothelial function,and may be a biological marker for evaluating endothelial function.2.The level of essential hypertension is correlated with serum Nrg4,and Nrg4 may be a target for the treatment of hypertension.