Expression And Clinical Significance Of C-Jun And C-Fos In Peripheral Blood T Cells Of Children With Relapsing Idiopathic Nephrotic Syndrome

BackgroundIdiopathic nephrotic syndrome（INS）is one of the common glomerular diseases in childhood.77.6%-91.0%of children are initially hormone-sensitive,but 80%-90%of children have relapses,or even frequent relapses.There is evidence that the abnormal expression of transcription factors in T cells is closely related to the recurrence of INS,but the specific transcription factors involved are still unclear.Because the transcription factor activator protein-1（AP-1）is closely related to the pathogenesis of INS in children,and AP-1 plays an important role in the chronic progression of a variety of kidney diseases,we speculate that the abnormal expression of AP-1 plays an important role in the recurrence of INS.This study will discuss the recurrence mechanism of INS from the perspective of whether the AP-1 components c-Fos and c-Jun are abnormally expressed.ObjectiveBy detecting the m RNA expression and protein expression levels of c-Fos and c-Jun in peripheral blood T cells during the initial remission and the initial relapse of INS children,we can explore whether the abnormal expression of AP-1 is related to the recurrence of INS.MethodsTwenty children with INS who were diagnosed in the Pediatric Inpatient Department of the First Clinical College of Xinxiang Medical University were selected.5ml of peripheral blood will be drawn during the initial remission period.During the follow-up,if there is a recurrence,5ml of peripheral blood will be drawn again during the initial relapse period.Use immunomagnetic beads to separate peripheral blood T cells,and use real-time quantitative PCR（RT-q PCR）,western blotting and other techniques to detect the m RNA expression and protein expression levels of c-Jun and c-Fos in T cells.ResultsRT-qPCR results showed that during the initial recurrence,the m RNA expression of c-Jun and c-Fos in the T cells of INS children were significantly higher than those in the initial remission period(27.88×10^-3±3.69×10^-3 vs 3.63×10^-3±0.48×10^-3,P<0.01;3.79×10^-3±0.45×10^-3 vs 0.55×10^-3±0.04×10^-3,P<0.01),the difference was statistically significant.Western blotting results showed that the protein accumulation of c-Jun and c-Fos in the above T cells was higher than that in the initial remission phase(53.10×10^-3±4.60×10^-3×10^-3 vs 14.53×10^-3±3.73×10^-3,P<0.01;367.75×10^-3±101.93×10^-3 vs 13.24×10^-3±3.37×10^-3,P<0.05),the difference was statistically significant,which confirmed the RT-q PCR results.ConclusionThe expression of AP-1 components c-Jun and c-Fos increased significantly during the recurrence period,suggesting that c-Jun and c-Fos play a certain role in the recurrence of INS in children.Periodic detection of AP-1 level changes during remission period may be able to predict the tendency of INS children to relapse to some extent.