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Active Components Of Activating Latent HIV From Wikstroemia Chamaedaphne Meisn.

Posted on:2022-10-13Degree:MasterType:Thesis
Country:ChinaCandidate:W H WangFull Text:PDF
GTID:2504306509462164Subject:Pharmacy
Abstract/Summary:PDF Full Text Request
This paper contains three parts.Part One:14 compounds were isolated from the branches and leaves of W.chamaedaphne using ODS column chromatography.Their chemical structures were determined by a variety of spectroscopic methods.14 compounds were identified and named as:XLSF05(1),XLSF06(2),simplexin(3),wikstchalide X(4),pimelea factor S6(5),pimelea factor S7(6),(+)-pinoresinol(7),phillygenin(8),acutissimalignan B(9),(-)-matairesinol(10),3,4-divanillyltetrahydrofuran(11),bis(2-ethylhexyl)phthalate(12),1-linolenoylglycerol(13),2-linolenoylglycerol(14).Compounds 1-5 are diterpenoids and 6-10 are lignans,and 1 is a new compound.Compounds 1-14 were isolated from this plant for the first time.Compounds 8,9,11-14 were isolated from this genus for the first time.Part Two:The total flavonoid of extracts from the buds of W.chamaedaphne was studied.1.The total flavonoid extracts were prepared and enriched by column chromatography from the buds of W.chamaedaphne.8 flavonoids were isolated from the extracts by silica gel column chromatography,sephadex LH-20 and semi-preparative HPLC,and their structures were identified as isoorientin(15),orientin(16),vitexin(17),isovitexin(18),quercetin 3-O-β-glucopyranoside(19),cynaroside(20),astragalin(21),luteolin-4’-O-glucoside(22).2.A HPLC method for simultaneously determining of 8 flavonoid compunds was established.The totol content of 8 flavonoid compunds was tested as(55.19±0.32)%in the flavonoid extracts using this method.The content of luteolin-4’-O-glucoside was(24.18±0.27)%,wchich is the mian flavonoid compund in the extracts.This HPLC method is simple,reliable and reproducible.3.The antioxidant activity of the flavonoid extracts were evalued in vitro.The FRAP values and the IC50of DPPH free radical-scavenging for the 8 flavonoid compounds and the flavonoid extracts were 0.19-0.84(?)mol/m L and 4.03-149.56(?)g/m L,respectively.Compoud cynaroside showed,which was better than that of Vc.This result reveals that the total flavonoid extracts displayed a strong antioxidant activity,which is an important medicinal substances in the bud of W.chamaedaphne.Part Three:The activating latent HIV activity of 22 compounds was tested in He La-NH2 cells with typical latent HIV model.The results showed that compounds 1-7,10,and 12-14 showed more than 5 times fluorescence intensity at 10(?)M,which was similar to the activity of prostratin.CCK-8 method was used to determine the cytotoxicity of 22 compounds.All compounds except 4 showed the cell survival rate≥90% at 10(?)M.
Keywords/Search Tags:Wikstroemia chamaedaphne Meisn, Diterpenoid, Total flavonoid extracts, HIV, LRAs
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