Changes Of Amino Acid Metabolomics In Preterm Infants With Different Growth Rates

[Objective]In order to understand the differences of plasma amino acid profiles in premature infants with different growth rates,the non targeted liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was used to detect the relative content of plasma amino acids,and to explore its relationship with growth and development and possible mechanism,so as to provide theoretical basis for individualized nutritional intervention in premature infants.[Methods]from March 2020 to January 2021,preterm infants with gestational age<34 weeks or birth weight<2000 g who were born in the obstetrics department of the First Affiliated Hospital of Kunming Medical University and transferred to the Department of Neonatology were selected as the subjects.According to fetal growth,they were divided into SGA group and non SGA group.According to different growth rates of premature infants during hospitalization,SGA group and non SGA group were divided into normal weight gain group and slow weight gain group respectively.A prospective non-randomized case study was conducted to determine the relative content of plasma amino acids in preterm infants within 72 hours after birth and at discharge by non targeted ultra performance liquid chromatography tandem mass spectrometry.Spss23.0 statistical software was used for univariate analysis and multivariate logistic regression analysis.[Result]1.Within 72 hours after birth,the relative contents of threonine,glycine,glutamine and alanine in SGA group were higher than those in non SGA group,and the difference between the two groups was statistically significant(P<0.05);At discharge,the relative contents of arginine and citrulline in SGA group were significantly higher than those in non SGA group(P<0.05),and there was no significant difference in other amino acids between the two groups(P>0.05).2.In SGA group,there was no significant difference in the relative contents of all amino acids detected within 72 hours after birth between the normal weight gain group and the slow weight gain group(P>0.05);At discharge,compared with the normal weight gain group,the relative content of histidine in the slow weight gain group was significantly higher(P<0.05),and there was no significant difference in other amino acids between the two groups(P>0.05).3.In the non SGA group,the relative contents of isoleucine,leucine,arginine,proline,glutamic acid,cysteine and cystine in the slow weight gain group were significantly higher than those in the normal weight gain group within 72 hours after birth(P<0.05),while the other amino acids had no significant difference(P>0.05);At the time of discharge,compared with the normal weight gain group,the relative content of cysteine in the slow weight gain group was significantly higher,the difference between the two groups was statistically significant(P<0.05),and the difference of other amino acids was not statistically significant(P>0.05).4.Multivariate logistic regression analysis showed that the relative contents of isoleucine(or=20.012,P=0.037)and cysteine(or=7.232,P=0.040)were the risk factors for slow weight gain.[Conclusion]There were differences in plasma amino acid metabolism between SGA group and non SGA group;The metabolic profiles of plasma amino acids in premature infants with different growth rates were different.2.The relative contents of plasma isoleucine and cysteine are significantly correlated with the slow growth rate of premature infants,which has the potential to be used as biomarkers for early prediction of abnormal growth rate of premature infants.