Changes Of Fatty Acid Metabolomics In Premature Infants With Different Growth Rates

ObjectivesThrough non-targeted ultra-high performance liquid chromatography-tandem mass spectrometry analysis technology,detect the relative content of plasma fatty acids in premature infants with different growth rates at birth and discharge,analyze and compare the differences in the relative content of fatty acids with different growth rates,and explore the relationship between fatty acid metabolism and growth rate relation.MethodsPremature infants(gestational age<34 weeks or birth weight<2000g)hospitalized in the Department of Pediatrics of the First Affiliated Hospital of Kunming Medical University from March 1,2020 to February 28,2021 were randomly included as research objects.Case data such as birth information,nutritional support during hospitalization,physical development indicators,and complications of premature infants were collected.According to the weight gain rate during hospitalization,they were divided into:slow growth rate group(weight gain rate<15g/kg.d)and normal growth rate group(weight gain rate≥15g/kg.d).Venous blood samples were collected within 72 hours of admission and upon discharge,and the relative content of plasma fatty acids was determined by ultra-high performance liquid chromatography-tandem mass spectrometry.Epidata3.1 was used to establish a database,and SPSS 25.0 was used for statistical analysis.The statistical methods used mainly included descriptive statistics and measurement data conforming to the normal distribution.Two independent sample t tests were used for comparison between the two groups.The measurement data of the state distribution are represented by the median and interquartile range[M(P25,P75)].P75 levels were divided into high relative content and low relative content,and binary Logistic regression analysis was used to explore the effects of different levels of fatty acid content on the growth rate of premature infants.Results1.A total of 80 premature infants were included in the study.There was no significant difference in the relative content of fatty acids between the normal growth rate group and the slow growth rate group.Compared with the slow growth rate group,the relative content of methyl pentadecanoate and octanoate in the normal growth rate group was higher at discharge,and the difference was statistically significant(Z:-5.93,P<0.05)and(Z:-2.23,P<0.05).2.An increase in the relative content of myristic acid at discharge was a risk factor for slow growth in premature infants(OR:6.234,95%CI:1.198,32.436,P<0.05).Conclusions1.There are differences in methyl pentadecanoate and octanoate in fatty acid metabolites in premature infants with different growth rates,and the relative content is higher in the normal growth rate group.2.Binary Logistic regression analysis showed that increased myristic acid is a risk factor for slow growth rate in premature infants,and the risk of slow growth rate in premature infants in the high level myristic acid group increased by 6.234 times.