The Associations Between Copy Number Variation In Triple Negative Breast Cancer Gene And Clinicopathological Characteristic On The Prognosis Of Patients

Objective:To understand the gene copy number variation(CNV)in triple-negative breast cancer tissues and adjacent tissues,and to explore the relationship between CNV and the clinicopathological characteristics and prognosis of patients with triple-negative breast cancer.Methods: From January 2014 to January 2016,86 patients with triple-negative breast cancer who underwent surgical treatment in the Cancer Hospital of Xinjiang Medical University were collected.The whole exome high-throughput parallel sequencing technology was used to detect the condition of CNV in the samples,and combined with clinical pathological data and follow-up records for comprehensive analysis.Results: As of January 2020,after 5 years of follow-up,the patient recurrence rate was 34.88%.In the 86 pairs of samples,the difference in CNV in the recurrence and metastasis group was significantly higher than that in the disease-free survival group,and the difference was highly statistically significant(P < 0.01).The recurrent metastasis group has the most frequent CNV amplification on chromosome 8,and the disease-free survival group has the most frequent CNV loss and heterozygous region on chromosome17.The 5-year survival rate of CNV patients on chromosomes 8 and 17 is higher than that of no In patients with CNV,the difference was statistically significant(P<0.05).Age,primary tumor size,lymph node metastasis,and TNM staging were linearly correlated with CNV(r=-0.87,0.92,0.94,0.83,P < 0.05).Cox multivariate regression analysis showed that CNV and TNM staging were independent factors affecting the prognosis of patients with triple-negative breast cancer(HR=0.456,1.927,P <0.05).Conclusion:The difference in cancer tissue and adjacent tissues of TNBC patients CNV is related to age,primary tumor size,lymph node metastasis and TNM staging,and affects its prognosis.