| Objective: To investigate the expression of ubiquitin carboxy-terminal hydrolase L1(UCHL1)、high mobility group box-1 protein(HMGB1)in serum and hippocampus of young rats with chronic epilepsy induced by pentylenetetrazole(PTZ),and the effects of glycyrrhizic acid(GA)and mouse nerve growth factor(m NGF)on it’s expression,and to explore the intervention effect of GA and m NGF on brain damage caused by epilepsy.Methods: 130 clean-grade Wistar male rat pups,21 days old were randomly divided into 4 groups: group A(normal control group,n=31),group B(untreated group,n=33),group C(m NGF group,n=33),group D(GA group,n=33).Mode of administration was intraperitoneal injection(IP).During the modeling period,group A was IP an equal amount of 9g/L normal saline per day,and the other groups were IP PTZ40mg/kg.Intervention was performed after 14 days of administration to establish a chronic epilepsy model.During the intervention period,groups A and B were IP an equal amount of 9g/L normal saline,group C was IP with m NGF 4ug/kg,group D was IP with GA 30mg/kg for 7 days.Each rat was observed for 30-60 min after administration and its behavioral performance was recorded.After the last intervention,9-10 pups were randomly collected from each group at 3h,24 h,and 72 h.The expression of UCHL1 in serum and left hippocampus and the concentration of HMGB1 in serum were detected by ELISA,and the content of HMGB1 in right hippocampus was detected by Western Blot.Results:1.Behavioral observation: Before each administration,young rats are free to eat and move independently.There was no obvious discomfort or death in group A.After intraperitoneal injection of pentylenetetrazole,mice in groups B,C,and D showed various degrees of seizures.After 14 days of kindling,the model of chronic epilepsy was established.2.ELISA test results: The test results of serum HMGB1 found: At each time point,the serum HMGB1 concentration in group B was the highest,group A was the lowest,and groups B,C,and D were higher than group A,the difference was statistically significant(all P = 0.000).Each time point of group C and D is lower than that of group B,there is a statistically significant difference between the two groups(P of the group C and group B = 0.000,0.000,0.007,P of the group D and group B = 0.000).Each time point of group C was higher than that of group D,there was a significant difference(P = 0.002,0.000,0.002).The test results of serum UCHL1 suggest: At each time point,the serum UCHL1 concentration in group A was the lowest,and group B was the highest.Group C and D were significantly lower than group B at each time point,the difference was statistically significant(P of the group C and group B = 0.003,0.001,0.013,P of the group D and group B = 0.000,0.001,0.000).The test results of hippocampus UCHL1 found: The change trend of hippocampal UCHL1 in each group of young rats at different time points is similar to the change trend of serum UCHL1.The hippocampal UCHL1 concentration in group A was the lowest,and group B was the highest.Groups B,C,and D were higher than group A.The difference was statistically significant(all P﹤0.05).Groups C and D were significantly lower than group B,and the difference was statistically significant(P of the group B and C = 0.000,0.000,0.001,P of the group B and D = 0.000).3.Western Blot test results: At each time point,the expression level of HMGB1 in the hippocampus tissue in group B was the highest,group A was the lowest,and groups B,C,and D were all higher than that of group A,the difference was statistically significant(all P = 0.000).Group C and D were significantly lower than group B at each time point,the difference was statistically significant(P of the group C and group B = 0.000,0.032,0.003,P of the group D and group B = 0.000).Group D was lower than group C at each time point,the difference was statistically significant(P = 0.020,0.000,0.003).Conclusions:1.HMGB1 and UCHL1 are highly expressed in epilepsy brain tissue and serum.Serum UCHL1 can be used as a biomarker for epilepsy-related brain injury.2.GA can inhibit the release and expression of HMGB1 to reduce inflammation,thereby effectively reducing brain damage caused by epilepsy.3.mNGF has a repairing effect on nerve damage caused by epilepsy and a protective effect on brain damage.4.GA is better than mNGF in inhibiting the release and expression of HMGB1. |
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