Analyse And Identify The Preparation,Structure Modification And Antibacterial Activity Of The Main Triterpenes In Rosemary Leaves

Rosemary is a plant with great edible and medicinal value.It is widely planted in Guangxi,Yunnan,Guizhou and Xinjiang.This subject adopts the structural differences in the system with target sample and impurity in the precise separation scheme design,in order to achieve the purpose of the separation of impurities.After the first time,the main triterpene acid component of the rosemary was identified as a high-performance liquid chromatographic peak,and the antibacterial activity of four pentacyclic triterpene acids was systematically studied for the first time.The amination of ursolic acid C3 site has not been reported in literature,the first in this paper.Firstly,four kinds of pentacyclic triterpene in Rosemary were extracted by ethanol ultrasonic,and the ursolic acid was separated from them.Three kinds of ursolic acid,oleanolic acid and white oleic acid were identified by high performance liquid chromatography(HPLC)by reference to relevant papers,and the last one was not reported in the literature,and it could not be separated from ursolic acid by general method.After using half preparation liquid chromatographic separation,high resolution mass spectrometry,nuclear magnetic resonance(NMR)spectrum methods,the fourth pentacyclic triterpene constituents identified as for MA.By comparing the structure of MA and ursolic acid,precise design for both structure olefinic bond in the amount of difference,by dealing with silica gel silver nitrate precast thin layer to achieve the purpose of separation.At the same time,the amount of silver nitrate was investigated.Through micro broth dilution method system evaluation of ursolic acid,oleanolic acid,white birch as fatty acid and MA of antibacterial activities.Eventually found ursolic acid compared to the other three have stronger antibacterial activity.For antibacterial experiment results,the innovative amination modification was carried out on the C3 of ursolic acid,by reducing ammoniation method to join contain amino side chain,and identified by using the method of nuclear magnetic resonance spectrum synthesis product.This series of modified products currently has not been reported in literature.Finally,in this thesis,the antibacterial activity of a series of ursolic acid derivatives was studied by microbroth dilution method and coating plate method.As a result,it was found that the antibacterial activity of the synthesized ursolic acid derivatives was enhanced to some extent,and it was proved that the structure modification direction was correct.