A Comparative Study Of Parathyroid Gland Autotransplantation Methods In Rabbits

Objective:In this experiment,four immediate parathyroid autotransplantation methods,namely,intramuscular pellet embedding,intramuscular homogenous injection and subcutaneous homogenous injection,were studied in rabbits to observe the transplantation effect,in order to further confirm the survival and regeneration of parathyroid glands after transplantation by the four methods from the histological point of view and to provide clinical data for reference.Methods:Thirty healthy adult rabbits were randomly divided into five groups,A,B,C,D and E.Group A was the control group,group B was the intramuscular pellet embedding group,group C was the subcutaneous pellet embedding group,group D was the intramuscular homogenous injection group and group E was the subcutaneous homogenous injection group.Four different methods were used to perform immediate autotransplantation of the inferior parathyroid gland on one random side of each group of rabbits.The duration of the surgical procedure was recorded separately from the time the parathyroid tissue was cut into pellets,the homogenate was made,and the processing of the isolated parathyroid gland was started to the time the transplantation was completed and marked with sutures for statistical analysis.Each group of rabbits after surgery was randomly divided into three groups,and the exposed and explored parathyroid glands of group A and the tissues of the transplantation sites of the remaining groups were stained with HE at the first,second and third weeks after transplantation to observe the viability and regeneration of transplanted parathyroid cells.Results:1.As observed under light microscopy,a higher proportion of lysed,necrotic parathyroid cells was seen in group B compared with group D at week 1 post-transplantation.At the 2nd week after transplantation,the proportion of residual necrotic and apoptotic parathyroid cells was higher in group B.Proliferating parathyroid cells were not yet observed,while proliferating parathyroid cells were already visible in group D.At the 3rd week after transplantation,the proportion of hyperplastic parathyroid cells in group D was significantly higher than that in group B.In contrast,there was no significant difference between groups C and E.We also observed that the homogenous injection method had more scattered distribution of transplanted parathyroid cells compared with the pellet embedding method,which could contact with the surrounding tissues more fully.In this study,all three groups,C,D and E,observed the generation of new blood vessels and the proliferation of parathyroid cells at the transplantation site at the 2nd week after surgery,and all four groups observed the proliferation of neovascularization and parathyroid cells at the 3rd week after transplantation.2.The results of multiple comparisons of transplantation time in each group were: the difference between the transplantation time in group B and group C was not statistically significant(P > 0.05),and the difference between the transplantation time in the remaining groups was statistically significant(P < 0.05),with groups B and C being the quickest and group E being the most time-consuming to operate.Homogenization time:(5.90±0.58)min;pellet making time:(2.21±0.73)min,the difference between them was statistically significant(t=13.754,P<0.05).Conclusion:1.All four transplantation methods were effective.2.The intramuscular homogenization injection method was more favorable to the survival of transplanted parathyroid cells compared to the intramuscular pellet embedding method,and there was no significant difference between the subcutaneous homogenization injection method and the subcutaneous pellet embedding method.The proliferation of subcutaneous transplanted parathyroid cells was similar to that of muscle transplants.3.The most time-consuming transplantation method was the subcutaneous homogenate injection method,and the muscle pellet embedding method and subcutaneous pellet embedding method were faster to operate.Pellet production is faster than homogenization.