The Research Of The Influential Factors Of The Cellular Viability In The Invitro Preservation During Parathyroid Autotransplantation

ObjectiveTo investigate the influential factors of parathyroid cellular viability in the invitro preservation during parathyroid autotransplantation, and to establish the best method of parathyroid tissues in the invitro preservation. So as to increase the survival rate of parathyroid autotransplantation through reducing the influence of the parathyroid cellular viability during the in vitro preservation.MethodsThis prospective study included12patients who underwent total thyroidectomy with the papillary thyroid carcinoma which infiltrated the parathyroid gland, from January to December,2014.12parathyroid glands infiltrated by the cancer were excised immediately, and then each intact parathyroid tissue was cut into7small pieces. One parathyroid fragment was fixed fresh and served as control. The remaining6fragments were kept in normal saline at room temperature (22-24℃) or DMEM solution at4℃for0.5hour,1.0hour, and2.0hour respectively. At the end of each period, the fragments were removed from the solution, fixed, and prepared for ultrathin sections to assess the ultrastructural integrity of the cell. ResultsAfter preservation in the normal saline at room temperature for0.5hour, the nuclear chromatin was condensed and a few of the mitochondria was swelling, while karyolysis, the chromatin condensed, intranuclear vacuoles, and mitochondria swelling were observed after1.0hour. After preservation in the DMEM solution at4℃for0.5hour, the ultrastructural findings were consistent with normal tissue fragments, while the nucleus was enlarged, mitochondria was swelling, and vacuoles were evident after1.0hour.ConclusionThe medium and time of preservation affect the cellular viability of the parathyroid gland during the parathyroid autotransplantation. The parathyroid tissue should be preserved in the cell culture solution at4℃during parathyroid autotransplantation, and should be no more than one hour. The parathyroid gland should be panted into the muscle as quickly as possible.