Study On Changes Of Acetylcholine Concentration In Serum And Muscle Of Rabbits After Poisoned By Bungarus Multicinctus Snake And The Timeliness Of The Bungarus Multicnctus Antivenin

Background:Snake bite is a serious global public health problem.As high incidence rate and mortality rate,snake bites will also bring heavy financial burden to the victims and society.There are ten species of highly venomous snakes in China,commonly known as"China’s Top Ten Viperes".The Bungarus multicinctus is a kind of neurovenomous snake.After being bitten,the local symptoms of the wound are mild,but within a few hours,the patient has a systemic poisoning reaction,mainly manifested as respiratory muscle paralysis.In severe cases,it can cause respiratory arrest and death with a high mortality rate.It is a common emergency and serious case in the emergency department.After the poisoning of the Bungarus multicinctus snake bite,the use of the Bungarus multicnctus Antivenin is the first choice for treatment.At present,the animal model of Bungarus multicinctus poisoning accorded with the clinical characteristics is not found.Therefore,by simulating the clinical case of the Bungarus multicnctus snakebite,a rabbit model of the Bungarus multicnctus poisoning that is initially constructed;And on the basis of this model,the timeliness of the Bungarus multicnctus Antivenin effect is further studied,which provide assistance to rescue measures for bite injury,reduce mortality,reduce hospital stay,improve prognosis,and improve the level of treatment for neurovenomous snakebite.Objective:The purpose of this study is to initially construct a rabbit model of Bungarus multicnctus intoxication that is more accorded clinical manifestations and to detect changes in the concentration of acetylcholine in rabbit serum and eyelid muscles after poisoning based on this model,and to further explore the Bungarus multicnctus Antivenin timeliness of action.Methods:Select New Zealand white rabbit,according to human rabbit equivalent dose conversion table and the Bungarus multicinctus one bite detox scale,prepare different concentrations of the Bungarus multicinctus snake venom solution.Completely simulate the clinical situation of the bite of the Bungarus multicinctus,subdivided into 12 groups according to the different injection concentrations of the Bungarus multicinctus snake venom,after the injection is completed,statistically analyze the time of apnea in each group of experimental rabbits,and repeat the experiment while reducing the injection Range,and finally screen out the target snake venom injection volume that meets the success of clinical performance modeling;After successful modeling,according to bite poisoning 0 minutes,1 minute,2 minutes,3 minutes,4 minutes,5 minutes,10 minutes and 30 minutes,1 hour,2 hours,3 hours,4 hours,5 hours and 6 hours for grouping,and at the same time take blood and eyelid muscle tissue samples at the corresponding time points in each group,and detect the content of free acetylcholine in each group sample by ELISA method to explore Whether the clinical characteristics and changes of acetylcholine concentration at different time points after the poisoning of the Bungarus multicinctus snake bite are consistent.According to the different injection time of the Bungarus multicnctus Antivenin,the rabbits were divided into 0 minutes group,10minutes group,30 minutes group,1 hour group,2 hours group,3 hours group,4hours group,5 hours group,6 hours group,blank control group and experimental control group,to explore the time effect of the Bungarus multicnctus Antivenin.Result:1.The average death time of rabbits in the 1/12 times snake venom content group（0.0179 mg/kg）is 5.92±0.22h compared with the average death time in rabbits 1/12.5 times snake venom content（0.0172 mg/kg）is 6.51±0.23,the difference is statistically significant,and finally determined that the target injection dose of the initially constructed rabbit model that is more in line with the clinical characteristics of the poisoning of the Bungarus multicnctus snake bite is a mixed solution of Bungarus multicnctus snake venom dry powder at0.0179 mg/kg and 0.6444 ul/kg H₂O.2.After successfully injecting the target dose of the Bungarus multicnctus snake venom,the free acetylcholine concentration of the rabbit serum began to decrease at 3 minutes;the free acetylcholine concentration in the eyelid muscle tissue began to increase at 3 hours.3.After successfully injecting the target dose of the Bungarus multicnctus Antivenin,each group was injected with the Bungarus multicnctus Antivenin.The blank control group,immediate group,10 minute group,30 minute group,1hour group and 2 hour group all survived;3 The hour group,4 hour group,5hour group,6 hour group and the experimental control group did not survive.Conclusion:1.With a mixed solution of 0.0179 mg/kg of the Bungarus multicnctus snake venom dry powder and 0.6444 ul/kg H₂O,a rabbit model that conforms to the clinical characteristics of the Bungarus multicnctus poisoning can be initially constructed.2.The disease progressed rapidly after the poisoning of the Bungarus multicnctus snake bite.The Bungarus multicnctus snake toxin can enter the blood system within 3 minutes and cause a poisoning reaction;after 3 hours,the toxin level of Bungarus multicnctus reach the peak through binding the receptor of Acetylcholine.3.The treatment effect was affirmative for the use of the Bungarus multicnctus Antivenin within 3 hours after the poisoning by the Bungarus multicnctus snake bite.