Protective Effects Of Rice-derived Bran Bioactive Peptide On Oxidative Stress In Endothelial Cells Induced By High Glucose

China is a big country of rice cultivation and consumption,and rice is one of the staple foods.Except for the refined white rice which is mainly eaten,the utilization rate of rich nutrients in the processed by-products is very low.Rice bran contains 64%nutrients of rice,in which the protein content is high,its amino acid composition is reasonable,and its allergy is low,so it has high value of development and utilization.With the change of modern diet,more and more people are obese.A long-term high glucose diet is one of the main causes of obesity,and high blood glucose concentration will increase the risk of diabetes and cardiovascular disease.Our team has isolated and screened an active peptide with excellent antioxidant ability from rice bran protein for in-depth study.Previous studies have shown that the Rice-derived bran bioactive peptide(RBAP)can enhance the ability of blood vessels to resist oxidative stress.On this basis,combining with the effect of high glucose on human body,the oxidative stress of endothelial cells induced by high glucose was taken as the research object to explore the antioxidant activity and mechanism of RBAP in high glucose oxidative injury,so as to provide guidance for the nutritional intervention of bioactive peptides in sub-healthy people with high blood glucose and those who need to strengthen blood glucose control through diet.The human umbilical vein endothelial cells(HUVEC)was induced by high concentration of glucose to establish oxidative stress model injury group,and RBAP was used as an effector to protect HUVEC to construct protective group.The results of Hype and Hoechst staining showed that RBAP could maintain the normal morphology of endothelial cells under high glucose injury;Flow cytometry showed that RB AP could reduce apoptosis and maintain normal cycle;At the same time,RBAP could maintain the stability of mitochondrial membrane potential,significantly reduce the content of ROS and MDA,increase the content of SOD and GSH,and improve the total antioxidant capacity(T-AOC).The results of tube formation and permeability of endothelial cells showed that RBAP was helpful for endothelial cells to maintain normal physiological function.In summary,it is suggested that RBAP can enhance the ability of endothelial cells to resist oxidative damage caused by high glucose.Further in-depth research on the anti-oxidant mechanism of RBAP,Western Blot results show that RBAP can inhibit activation of the AGEs-RAGE pathway caused by high glucose,reducing NADPH oxidase causes the excessive production of ROS by raising RAC1,thereby inhibiting the activation of inflammatory pathways NF-?B and JNK pathways.Reducing the amount of protein expression of the downstream factor EGR-1,PAI-1,and VEGF,in which the protective effect of endothelial cell oxidative stress caused by high glucose is achieved.After preliminary determination of the mechanism of RBAP,combining the result that high glucose will promote the occurrence and development of typical cardiovascular disease atherosclerosis,the research has a in-depth exploration of the effect of RBAP on the risk of atherosclerosis under high glucose conditions.The bioinformatics analysis of atherosclerosis is carried out through the comprehensive gene expression database GEO,STRING,the Human Protein Reference Database(HPRD)and the Database for Annotation,Visualization and Integrated Discovery(DAVID)database.Analyzing and predicting risk genes through PPI,Data models such as GO and KEGG,which screen out the key factors related to oxidative stress include ICAM1,IL1B,TLR8,CDC23,DTX3L,etc.;Key pathways include JAK-STAT,PI3K-Akt,MAPK,cytokine regulation and Cell morphology control,etc..Meanwhile,it verified the effect of RBAP on the expression of key factors ICAM-1 and IL1B under high glucose conditions,which showed that RBAP slowed down the occurrence and development of atherosclerosis in cardiovascular diseases under the condition of high glucose.In summary,RBAP can protect endothelial cells from oxidative stress damage caused by high glucose and may reduce the risk of atherosclerosis under high glucose.