The Research On The Effect Of Rice Bioactive Peptides Have On NF-κB Signaling Pathway Of Oxidative Injured Endothelial Cells

Rice resource contains luxuriant bioactivator, which has functions of good antioxidant, lowering blood pressure and improving immunity. Rice bioactive peptides (RBP) can be divided into rice peptides of antioxidant activity, rice peptides of antihypertensive, rice flavor peptide, rich immune bioactive peptide and opioid peptides according to the functional effects. This group has been devoted to the study of antioxidant function and action mechanism of rice bioactive peptides, in the previous study we found that rice bioactive peptides in hydrogen peroxide (H2O2) induced human umbilical vein endothelial cells (HUVEC) in oxidative stress have good inhibitory effect on the system, this is not only reflected in the cell level, there are also obvious differences in the protein level.Therefore we speculate that rice bioactive peptide can regulate the stimulation of certain signaling pathways to abtain the effect of inhibiting the oxidative stress. This paper introduces the common injury mechanism of vascular endothelial cells, signaling pathways which are involved in different damage system, focusing on the oxidative stress related signal pathway. Based on above research, through 70 μmol/L H2O2 induced HUVEC, with the RBP of 0.2 mg/ml preprocessing 24 hours and Western blot detection of expression changes of vascular endothelial cells apoptosis related factor NF-κB, p38MAPK, IKKβ before and after activation in H2O2 induced oxidative stress damage and before and after the protective effect of rice bioactive peptides in the process. These are all to explore effects of RBP on NF-κB signaling pathway of oxidative injured endothelial cells.Experimental results showed that expression of the NF-κB protein and p-NF-κB protein, p38MAPK protein and p-p38MAPK protein, IKKp protein and p-IKKβ protein in H2O2 damage model group (70 μmol/L) increased significantly than normal control group. After the pretreatment of rice bioactive peptides (0.2 mg/ml) for 24 hours, expression of the NF-κB protein, p38MAPK protein and p-p38MAPK protein, IKKβ protein in every group decreased significantly than H2O2 damage model group (70 μmol/L); there are few differences between the expression lever of p-NF-κB protein in H2O2 damage model group (70 μmol/L) and protective group of rice bioactive peptide; the expression lever of p-IKKβ protein in protective group of rice bioactive peptide is higher than in H2O2 damage model group (70 μmol/L).The results showed that the protective mechanism of rice bioactive peptide on oxidative damage of endothelial cells and down-regulation expression of NF-κB protein, p38MAPK protein, p-p38MAPK protein and IKKβ protein in NF-κB signaling pathway are closely related. By inhibiting expression of apoptosis related factors to regulate the ability of antioxidant injury of human umbilical vein endothelial cells, rice bioactive peptide antioxidantly protect the injury of human umbilical vein endothelial cells. The study found that after activation of NF-κB protein and IKKβ protein phosphorylation, rice bioactive peptides play a role in up-regulating the increasing expression of p-NF-κB and p-IKKβ caused by H2O2. The results of the study show that there are differences before and after activation of NF-κB protein and IKKβ protein of oxidative damage endothelial cells, the regulation effect of rice bioactive peptide on NF-κB and IKKβ protein before and after activation is not the same. This will provide a favorable theoretical basis for the development of rice bioactive peptide as the antioxidant functional food and polypeptid drugs.