Research On The Integration Of Processing And Processing In The Origin Of Polygonatum

Objective:The purpose of this article is to establish the quality standard of Rhizoma Polygonatum decoction pieces by establishing the quality evaluation system of Rhizoma Polygonatum decoction pieces,optimizing the parameters of the integrated processing of Raw Rhizoma Polygonatum,steamed Rhizoma Polygonatum and Alcohol Rhizoma Polygonatum and comparing the difference between the traditional method and the integrated method.It also aims at providing references for the Effectiveness,safety,and quality control studies of the Rhizoma Polygonatum and promoting the further development and utilization of Rhizoma Polygonatum.Methods:1.The establishment of the quality evaluation system:(1)evaluation criteria for the appearance characteristics of Rhizoma Polygonatum decoction pieces;(2)identification of Rhizoma Polygonatum thin layer chromatography;(3)establishment of fingerprint of Rhizoma Polygonatum hplc;(4)The establishment of a method for determining the content of moisture,extracts,polysaccharides,total saponins and5-hydroxymethyl furfural;2.The optimization of the integrated processing of Raw Rhizoma Polygonatum,Steamed Rhizoma Polygonatum and Alcohol Rhizoma Polygonatum;3.a comparative study of the integrated method and the traditional method of the processing of Rhizoma Polygonatum;4.The establishment of the quality standards of Rhizoma Polygonatum.;5.A Research on composition changes of3 processed ingredients of the Rhizoma Polygonatum based on GC-MS.Results: 1.The establishment of a quality evaluation system:(1)Establishing evaluation criteria for the appearance characteristics of Raw Rhizoma Polygonatum,Steamed Rhizoma Polygonatum,and steamed yellow rhizome of the outer skin color,center color,cut surface,texture,and odor as indicators.(2)Oleic acid was used as a reference to establish a Rhizoma Polygonatum thin layer chromatography identification method,which has the advantages of clear spots and high resolution.(3)HPLC fingerprints were established using 10 batches of Rhizoma Polygonatum medicinal materials to determine 9 common peaks.(4)Determine the drying method to determine the moisture content,the reflux method to determine the extract,the ultraviolet spectrophotometer to determine the polysaccharides and total saponins,and the HPLC method to determine the 5-hydroxymethylfurfural.2.The best processing method for Raw Rhizoma Polygonatum was as follows: take fresh and pure Rhizoma Polygonatum,cut into 6mm thick tablets,then dry at 60 ℃;the best processing technology of Steamed Rhizoma Polygonatum is: take fresh and purified Rhizoma Polygonatum,steam for 10 h,take out,dry at 70 ℃,then steam for 8h,take out,cut into 10 mm thick slices,and dry at 70 ℃ to get;The best processing technology of Alcohol Rhizoma Polygonatum is: take the freshly prepared Rhizoma Polygonatum,steam it for 10 h,then take it out and dry it at 70 ℃,add 20% chinese yellow rice twine,moisten it for 10 h,steam it for 8h,then take it out,cut it into 10 mm thick slices,and dry it at 70 ℃ to get,3.The t test of the polysaccharides,extracts and total saponin content of the Raw Rhizoma Polygonatum decoction pieces obtained by the traditional method and the integrated method was performed respectively.The p-values were 0.934,0.388,and 0.919,all of which were greater than 0.05,which means there was no difference.The polysaccharides,extracts,total saponins,and5-HMF contents of the Steamed Rhizoma Polygonatum decoction pieces obtained by the traditional method and the integrated method were respectively tested by t-test,and the p-values were 0.246,0.017,0.886,0.501,all of which are greater than 0.05 except for the extract,that is,there is no significant difference in the content other than the extract,and the content of the Steamed Rhizoma Polygonatum extract obtained by the integrated method is higher than that of the traditional method.The contents of polysaccharides,extracts,total saponins,and 5-HMF in Alcohol Rhizoma Polygonatum decoction pieces were respectively tested by t-test,and the p-values were 0.802,0.371,0.946,and 0.856,all of which were greater than 0.05,which means there was no difference.4.The establishment of quality standards for Rhizoma Polygonatum.5.GC-MS was used to determine the chemical composition of Polygonatum sibiricum and its three processed products,and a total of 71 compounds were identified,mainly including alkane,olefin,aromatic hydrocarbon,ketones,esters,alcohols,etc.Among them,42 compounds were detected in Polygonatum sibiricum,44 compounds were detected in Raw Rhizoma Polygonatum,50 compounds were detected in Alcohol Rhizoma Polygonatum,and 41 compounds were detected in Steamed Rhizoma Polygonatum.Also,2,2-dimethyltetrahydrofuran,2,3-dihydro-3,5dihydroxy-6,Methyl-4(H)-pyran-4-one,which are characteristic of Maillard reaction,were founded in the processing.It indicated that beauty German reaction really happened in the processing of Rhizoma Polygonatum.Conclusion: This study established the evaluation criteria for the appearance characteristics of Rhizoma Polygonatum decoction pieces,the TLC identification method of Rhizoma Polygonatum decoction,and the HPLC fingerprint method.It also optimized the three integrated preparation processes of the decoction pieces and formulated the quality standards for decoction pieces.The differences in the components of the three Rhizoma Polygonatum tablets were compared using GC-MS,which provided ideas for the quality control and safety demonstration studies of Rhizoma Polygonatum.