Screening Of Enzyme Producing Resistance Strain From Chinese Medicine Residues Such As Salviae Miltiorrhizae And Its Resource Utilization

Chapter One,Literature ReviewTaking Salviae miltiorrhizae as an example,the utilization status of root and rhizome medicinal materials in the deep processing of medicinal materials is summarized.The effective constituents of tanshinone and salvianolic acid extracted by deep processing are often used as clinical preparations.The utilization of Salviae miltiorrhizae residues discarded in the industrialization process can be divided into two aspects:extraction of target components and direct utilization of nutrient components.Chinese medicine residues,which are mainly root and rhizome wastes,are mostly utilized by fertilizer,feed and matrix,or converted into components in residues to produce new energy.At the same time,the composition of cellulose and related cellulase applications are briefly described.Chapter Two,Screening of Resistant Strains Tolerant to Salviae Miltiorrhizae Residues and Evaluation of Cellulase Production AbilityIn order to realize the high-value utilization of Chinese medicine residues biotransformation,such as Salviae miltiorrhizae,Sophorae flavescentis and Glycyrrhizae are selected as research objects to produce high value-added cellulase.A biological method is used to screen the residue-tolerant strains.Using the bacteriostatic component tanshinone in the residue of Salviae miltiorrhizae to obtain a firngus,Penicillium expansum SZ13.The optimum fermentation environment parameters are obtained by optimizing the enzyme production process,and the peak enzyme production period of SZ13 is confirmed.The enzyme production ability of SZ13 in degrading different types of residues is investigated,and the results showed that S213 has high enzyme activity and strong stability in biodegrading various types of Chinese medicine residues.Chapter Three,Methodological research and application practice of enzyme production by mixed bacteria fermentationIn order to improve the utilization rate of cellulose resources in Chinese medicine residues,the enzyme production method is optimized by compounding strains.Chinese medicine residue is divided into bacteriostatic residues and general residues according to the presence of bacteriostatic components.Two types of mixed fermentation,tolerant strain compounding and compound enzyme method,are provided respectively.And their methodological investigation and verification are carried out.The results showed that the two mixed bacteria fermentation methods are consistent with the optimal fermentation parameters.The two methods are applied to enzyme production from different types of residues,and it is found that the compound strains have strong environmental adaptability,good synergistic growth and enzyme production effect.Chapter Four,Application of cellulase in saccharification of Astragalus stems and leaves and discussion of enzymatic characteristicsIn order to weaken the physical barrier for the dissolution of components in the residues,cellulase is used to degrade the Astragalus stems and leaves for saccharification,and the enzymatic properties are also discussed.In this study,cellulase enzymatic hydrolysis and strain fermentation degradation are used to saccharify the Astragalus stems and leaves in their respective optimal environmental parameters.It is found that both methods could effectively increase the total sugar coltents in stems and leaves and increase the nutritional value of them.In order to utilize cellulase reasonably and efficiently,the enzymatic properties of cellulase produced by S213 are studied.the optimal temperature and pH range of cellulase are determined.The environmental stability of cellulase is also investigated,and the advantages of cellulase such as short-distance transportation and medium-term storage are confirmed.Using strains to ferment Chinese medicine residues to produce high-value-added products cellulase,while improving the dissolution rate of other components,has scientific value and practical significance for the utilization and industrialization of Chinese medicine residues.