| Objective: To investigate the correlation between single nucleotide polymorphism(SNP)of FOSL2 gene and persistent allergic rhinitis(PER)induced by dust mites in Jiangsu and Anhui Han populations.Methods: A total of 630 cases in the case group and 696 cases in the control group were included in a case-control study.Peripheral blood of subjects was collected for serum total IgE and allergen-specific IgE measurement,and peripheral blood of subjects was stored about 5ml with EDTA anticoagulant tube for DNAextraction.Questionnaire survey was conducted for all subjects,and general information of subjects was collected.According to the principle of linkage imbalance HapMap database also met the requirements of minimum allele frequency(MAF)>0.05 and γ2>0.08 of Chinese Han population.Combined with the functional prediction of SNPinfo Web and related references,five tag single nucleotide polymorphisms(tagSNP)sites of FOSL2 gene were screened out for TagMan genotyping.Results: There was no significant difference either in age(median 23 yrs,quartile 14~34 yrs)between the case group and the control group(median 26 yrs,quartile 14~34 yrs)(P=0.314),nor in gender(P=0.237).The serum total IgE was 187.615(74.78,407.250)IU/L in the case group and 29.670(13.185,59.135)IU/L in the case group,and the difference was statistically significant(P<0.001).After age,gender and other factors were corrected by Logistic regression,it was found that the frequency of rs9309662 in FOSL2 gene between the case group and control group was statistically significant.The comparison of TT between pure and mutant CC of this gene and wild pure and mutant was P<0.001,OR=4.214862,95%CI=2.374553-7.481433.In addition,the difference of the additive model(P=0.008,OR=1.276,95%CI=1.064-1.529)and the recessive model(P<0.001,OR=3.051,95%CI =1.819-5.117)in the case group and control group was also statistically significant.Both of them increased the risk of PER induced by dust mites in the Han population of Jiangsu and Anhui provinces.Stratified analysis of the subjects by gender showed that the difference of CC genotype frequency at rs9309662 between the case and control groups in male was statistically significant(P=0.011,OR=2.705,95%CI=1.251-5.850).Meanwhile,the additive model of this locus(P=0.045,OR=1.351,95%CI= 1.007-1.813)and the recessive model(P=0.013,OR=2.607,95%CI=1.220-5.572)were also statistically significant.In female,rs9309662 CC genotype frequency in same statistically significant differences between the cases and control groups(P=0.025,OR=3.356,95% CI=1.168 9.644).Meanwhile,difference in the implicit model between the case and control groups was also statistically significant(P=0.012,OR=3.778,95% CI=1.332 10.710).Haplotype analysis of rs9309662 and rs7562 of Fosl2 gene showed that the difference of C-T genotype frequency between the case group and control group was statistically significant(P<0.001,OR=3.336,95%CI= 2.020-5.512),indicating that the C-T genotype of rs9309662-rs7562 might increase the risk of PER.Conclusion: The SNP locus rs9309662 homozygous mutant CC of Fosl2 gene increased the risk of PER sensitization by dust mites in the han population in jiangsu and anhui provinces.Stratified analysis by sex showed that CC/CC+TC genotype at rs9309662 significantly increased the risk of PER in both male and female.Haplotype analysis showed that the c-t genotype of rs9309662-rs7562 significantly increased the risk of PER.Rs9309662 is located in the intron region of Fosl2,which may affect the transcription of Fosl2 gene,and its molecular mechanism and signal transduction pathway need to be further studied. |
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