Effect Of Shenqi Compound On Improving The Iatrinsic Immunemacrophage Surface Receptors In Diabetic Thoraeic Aorta By Positive Airway Inhibiting Metabolic Memory

Objective: This study is based on the "righteousness" theory of the motherland medicine,using systemic biology methods to demonstrate the general condition and weight of GK rats during the process of inhibiting the development of diabetic macroangiopathy by Shenqi compound.Fasting blood glucose,pathological observation of thoracic aorta hematoxylin-eosin staining,oxidative stress index oxidized low density lipoprotein(ox-LDL),Apoptosis index,innate immune macrophage surface scavenger receptor A(SR-A),Toll-like receptor 4(The role of TLR-4)and macrophage migration inhibitory factor(MIF)is mainly focused on the regulation of immune inflammatory microenvironment in large blood vessels.It is suggested that the application of Yangyin Yiqi Huoxue method can reduce blood sugar levels in rats.Repair and protect damaged vascular endothelium,inhibit oxidative stress on vascular injury,and explain the influence of innate immune macrophage surface receptor on diabetic macrovascular,and expand the thinking for clinical prevention and treatment of diabetic macroangiopathy.Methods: One hundred GK rats were randomly divided into five groups: model group,western medicine group,high-dose Chinese medicine group,middle-medication medium-dose group,low-dose Chinese medicine group,and adaptive feeding for one week(7 days).The high-fat diet was continuously fed for 4 weeks to simulate the metabolic memory process to create a model of macrovasc ular disease.After successful modeling,they were randomly divided into model group(W group),western medicine group(W group),Shenqi compound high-dose group(H group),Shenqi compound middle-dose group(Z group),and Shenqi compound low-dose group.(L group),each group of 20,another 20 normal wistar rats were fed as a control group(group C),a total of 6 groups.Each gro up was given the corresponding drugs,and the model group and the normal group were given distilled water for a period of 12 weeks(84 days).At the end of the 12th week,the experimental an imals were sacrificed.The general conditions,biochemical indicators of glucose metabolism,oxidative stress index ox-LDL,and thoracic aorta were taken for HE staining.DeadEndTM fluorescence assay TUNEL method was used to observe the average fluorescence inte nsity of endothelial cells and cell withering.In the case of death,the pathological morphology of the aorta was observed by light microscopy.The macrophage surface receptor scavenger receptor A,Toll-like receptor 4 and MIF immunocytokine were detected by ELISA.Results: 1.Successfully constructed a model of metabolic memory macrovascular disease in GK rats: Compared with the control group,the rats in the model group had extremely poor mental state,slow weight gain,and markedly elevated blood glucose,and the macrophage scavenger receptor SR-A.TLR-4,serum ox-LDL,serum MIF levels increased and higher than the control group,thoracic aortic endothelial injury,apoptosis,and apoptotic rate increased significantly.After treatment,the mental state of GK rats in the three groups of Shenqi compound gradually recovered,the coat color was lustrous,and the blood glucose showed a downward trend.It was better than the model group.The thoracic aortic endothelium injury recovered to normal,the apoptosis decreased,and the a poptosis rate decreased significantly..Shenqi compound can reduce the levels of macrophage scavenger receptor SR-A and TLR-4,affect the intrinsic immunity upstream expression of diabetic macroan giopathy,control the further development of inflammation;reduce the oxidative stress index serum ox-LDL level,effectively reduce the giant Apoptosis of phagocytes inhibits the metabolic memory effect of diabetes.Shenqi compound can lower serum MIF levels and inhibit the development of diabetic macroangiopathy.Conclusion: Shenqi compound can effectively improve the gen eral condition of GK rats,reduce the blood glucose of FG in GK rats,control the weight growth,reduce the levels of serum ox-LDL and MIF,protect and repair damaged vascular endothelium,reduce apoptosis and reduce The expression of innate immune macrophage surface receptors SR-A and TLR-4 reduces the development of vascular endothelial inflammation and effectively prevents the devel opment of diabetic macroangiopathy.