Font Size: a A A

Study On The Expression Of Cdc42、N-WASP、Arp2/3 In Peripheral Blood Of Patients With DRE

Posted on:2020-10-17Degree:MasterType:Thesis
Country:ChinaCandidate:C S GuoFull Text:PDF
GTID:2404330590964572Subject:Neurology
Abstract/Summary:PDF Full Text Request
Object To investigate the pathogenesis of drug-resistant epilepsy by examining the expression of mRNA and protein of Cell Division Cycle 42 GTP-binding protein(Cdc42),Neural Wiskott-Aldrich Syndrome Protein(N-WASP)and Actin-related protein 2/3(Arp2/3)in peripheral blood of patients with Drug-Resistant Epilepsy(DRE)and analyze the clinical value of Cdc42,N-WASP and Arp2/3 as objective indicators for predicting DRE.Methods 72 essential epilepsy patients who were attended at outpatients and inpatients in the Department of neurology of the Affiliated Hospital of Youjiang Medical University for Nationalities were selected from October 2016 to October 2018.According to the 2010 International League Against Epilepsy’s definition of Drug-Resistant Epilepsy,72 essential epilepsy patients were divided into 2 groups:32 patients with DRE were defined as DRE group,40 patients with drug well controlled were defined as the well controlled group.32 healthy persons were selected as healthy persons group.The expression of mRNA and protein of Cdc42、N-WASP and Arp2/3 in peripheral blood were measured by Quantitative real-time PCR(RT-qPCR)and Western Blot(WB).Experimental data were analyzed by ANOVA or rank-sum test.Considering of the common risk factors of DRE,we explored the effects of different drug administration,different disease course and different seizure frequency before medication on the expressions of Cdc42,N-WASP,Arp2/3.Results(1)Compared with well-controlled group and healthy persons group,Cdc42,N-WASP,Arp2/3 in DRE group were significantly increased,the differences were statistically significant(P<0.05).Compared with the healthy persons group,Cdc42、N-WASP、Arp2/3 in well-controlled group were significantly increased,with statistically significant differences(P < 0.05).(2)Comparative by different medical methods: Compared with the two drugs treatment effective group and monotherapy effective group,Cdc42,N-WASP,Arp2/3 in the invalid group of two drugs were significantly increased,and the differences were statistically significant(P<0.05);Compared with monotherapy effective group,Cdc42,N-WASP,Arp2/3 in the two drugs combined treatment effective group were increased,but the differences were not statistically significant(P > 0.05).(3)Comparative by different courses: compared with the subgroup with duration <5 years,Cdc42,N-WASP,Arp2/3 in the group with duration ≥5 years were significantly increased,the differences were statistically significant(P< 0.05).(4)Comparative by different seizure frequency before treatment: compared with the group with seizure frequency <4 times/month before treatment,Cdc42,N-WASP,Arp2/3 in the group with seizure frequency ≥4 times/month before treatment were significantly increased,the differences were statistically significant(P<0.05).Conclusion: The expression of Cdc42、N-WASP 、Arp2/3 in peripheral blood of patients with DRE significantly increase,which are higher in patients who were resistant to two drugs,having longer course and higher frequency of seizures,revealing that Cdc42,N-WASP,Arp2/3 in peripheralare blood are closely related to the occurrence and development of DRE,and used as indicators in peripheral blood predicting the occurrence of DRE.
Keywords/Search Tags:Drug resistance epilepsy, Cdc42, N-WASP, Arp2/3, Peripheral blood, pathogenesis
PDF Full Text Request
Related items