Benign Infantile Convulsions With Mild Gastroenteritis Etiology And Serum Inflammatory Factors Analysis

Objective 1.To detect fecal enterovirus in Benign infantile convulsions with mild gastroenteritis(BICE)and to understand the distribution of the pathogen of the disease in our region,so as to provide experimental basis for regional prevention and treatment.2.To detect the levels of IL-6 and TNF-α in the serum of BICE children and analyze the correlation between IL-6,TNF-α and the frequency and duration of convulsions in BICE children,and to speculate whether there is immune regulation dysfunction involving cytokines in BICE children.3.To know whether there is difference in WBC number and Hb content of blood routine between BICE children and mild gastroenteritis children without convulsion attack,and to speculate whether WBC and Hb of blood routine have correlation with BICE.Methods 1.From May 2017 to May 2018,30 cases of mild gastroenteritis with benign infantile convulsions hospitalized in the pediatric internal medicine ward of our hospital were selected as the experimental group,while 30 cases of mild gastroenteritis without convulsions with balanced age,gender and experimental group were selected as the control group.2.Detection of rotavirus in stool of two groups of children by colloidal gold method;Norwalk virus、echovirus and coxsackie virus were detected in stool of two groups of children by fluorescence PCR.3.The levels of serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in two groups of children were detected by chemiluminescence method.4.The blood count of WBC and hemoglobin(Hb)in the two groups were compared and analyzed.Results 1.In the experimental group,14 cases(46.67%)were rotavirus positive,2 cases(6.67%)were Norwalk virus positive,and 3 cases(10%)were Coxsackie virus positive.In the control group,11 cases(36.67%)were rotavirus positive,4 cases(13.33%)were Norwalk virus positive,1 case(3.33%)was echovirus positive,and 1 case(3.33%)was coxsackie virus positive.2.The levels of serum IL-6 and TNF-α in BICE group were higherthan those in control group,and the difference was statistically significant(P <0.05).3.In BICE group,there were 11 children with seizures ≥ 2 times and 19 children with seizures < 2times.The levels of serum IL-6 and TNF-α in children with convulsion ≥ 2 times were higher than those in children with convulsion < 2 times,and the difference between the two groups was statistically significant(P<0.05).4.In BICE group,there were 8 children with convulsion duration ≥ 5 min and 22 children with convulsion duration < 5 min.The levels of serum IL-6and TNF-α in children with convulsion duration ≥ 5 min were higher than those in children with convulsion duration < 5 min,the difference between the two groups was statistically significant.(P<0.05).5.There was no significant difference in WBC and Hb contents between the two groups(P>0.05).Conclusion 1.Rotavirus infection is the main pathogen of BICE children in this region.2.Children with BICE have a certain degree of immune regulation dysfunction.IL-6 、 TNF-α may participate in the convulsion process of BICE,and are correlated with the frequency and duration of convulsion.3.In this study,WBC and Hb of blood routine are not related to BICE convulsion and need further confirmation by large-scale experiments.