| Background:Psoriasis is a chronic,recurrent,refractory erythematous squamous skin disease,the cause of which is still unknown.Psoriasis is not only a skin disease,but also can seriously damage the physical and mental health of patients,patients will be associated with joint pain and deformity even lead to dysfunction,so that patients lose the ability to work and self-protection.Hypermetabolic syndrome,thyroid dysfunction and heart disease may also occur.Therefore,it is important to study the pathogenesis of psoriasis and find rational clinical treatment.One of the pathogenesis of psoriasis is the imbalance of Th17 / Treg,and Th17 and Treg cells also play an important role in C.albicans colonization/infection.Then,whether Th17,Treg,IL-23 and il-17 have a certain effect on the C.albicans colonization in the oral cavity of PsV patients,and whether their changes will increase or decrease the C.albicans colonization or infection in the oral cavity of PsV patients is not clear at present.This topic from psoriasis no candida engraftment,healthy people have candida engraftment four state,the preliminary discussion of Th17 and Treg,IL-17,IL-23 expression level,clear the relationship between the psoriasis with candida and Th17,Treg,IL-17,IL-23 to its influence,to guide the future of the emerging clinical application to provide some theoretical basis.Objective:1.Preliminary understanding of oral candida colonization in PsV patients;2.Discuss the role of Th17/Treg cells,IL-17 and IL-23 in the colonization of PsV and candida;3.To preliminarily understand the relationship between PsV and oral candida colonization.Methods:The clinical cases were from patients diagnosed with PsV in the department of dermatovenereal diseases of the second hospital of shanxi university from September 2018 to December 2018,and the control group was from healthy people in the physicalexamination center of the second hospital of shanxi university.Acquisition of PsV group and the control group respectively 30 copies of oral buccal mucosa and blood specimen of microscopic examination of the oral buccal mucosa specimens of fungi,SAD fungi cultivation,CHROMagar candida color medium,API20 C AUX candida strains of morphology and molecular biology identification of identification system,peripheral blood of Th17 / Treg cells expressing quantity using Flow cytometry instrument(Flow cytometer),IL-17,the expression of IL-23 enzyme linked immunosorbent test(ELLSA)for testing,SPSS21.0 was used for statistical analysis of the data.The statistical description of measurement data conforming to normal distribution was expressed by mean standard deviation,and the description of counting data was expressed by frequency and percentage.T-test or anova was used for the comparison between measurement groups in line with normal distribution,and chi-square test was used for statistical analysis for the comparison between counting data groups.Results:1.A total of 16 candida strains were obtained from 30 oral and buccal mucosa specimens of PsV patients,including 12 strains of C.albicans strain(40%)and C.glabrata4 strain(12.33%).A total of 10 candida strains were obtained from 30 oral and buccal mucosal specimens of healthy people,including 6 strains of C.albicans(20%),10 strains of C.glabrata3(10%),and 1 strain of C.krusei1(3.33%).2.The expression levels of Th17(21.13±5.10 vs7.05±2.64/μL,t=13.425),IL17(212.96±36.92 vs122.89±15.49 pg/ mL,t=12.322)and IL23(220.76±22.46vs138.88±19.35 pg/ mL,t=15.12)in PsV patients were higher than those in healthy subjects,P<0.05,indicating a significant difference.The expression level of Treg in PsV patients(24.93±7.43 vs39.39±13.38/μL,t=-5.17)was lower than that in healthy people,P<0.05,indicating a significant difference.3.The expression levels of Th17(5.59±2.18 vs7.79±2.58/μL,t=-2.314),IL17(103.25±5.18 vs132.71±6.98 pg/ mL,t=-11.779)and IL23(116.70±7.25 vs149.97±12.57pg/ mL,t=-7.711)in healthy oral population colonized by candida were lower than those in healthy oral population colonized by candida,P<0.05 was significant difference.Treg expression level(49.24±13.34 vs34.47±10.60/μL,t=3.303)in healthy population with oral candida colonization was higher than that in healthy population without oral candida colonization,P<0.05,indicating a significant difference.4.Th17(F=111.101,),Treg(F=24.213),IL17(F=170.491),and IL23(F=393.595)of PsV patients with or without oral candida colonization and healthy population were compared respectively,and P<0.05 was statistically significant,that is,significant differences were found between the four groups.To compare the expression levels of Th17,Treg,IL-17 and IL-23 between PsV patients with candida colonization and healthy people with candida colonization and PsV patients without candida colonization.It was found that Th17(17.89±4.32 vs5.59±2.18 vs24.82±2.99/μL),IL-17(186.44±14.76 vs103.25±5.18vs243.26±30.59 pg/ mL)and IL-23(201.74±8.22 vs116.70±7.25 vs242.50±9.68 pg/ mL)of PsV patients with candida colonization were all lower than those with candida colonization.However,it was significantly higher than that in PsV patients without candida colonization,P<0.05.Treg(30.37±4.27 vs49.24±13.34 vs18.72±4.91/μL)of oral cavity patients with candida colonization in PsV patients was higher than that of healthy population with candida colonization,but lower than that of PsV patients without candida colonization,P<0.05,indicating a significant difference.Conclusion :1.In the oral cavity of PsV and healthy population in this region,C.albicans is the most important colonizing bacteria,followed by C.glabrata.2.The increase of Th17,IL17 and IL23 and the decrease of Treg cells can promote the occurrence and development of PsV.3.The increase of Th17,IL17 and IL23 and the decrease of Treg cells can protect the body and reduce the colonization of candida.4.For this result,Candida colonization may not have a significant effect on the development of psoriasis vulgaris. |
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