| Objective:1.To study the distribution of serum HBV DNA level and HBV genotype in pregnant women with chronic hepatitis B virus carriers(AsC).2.To study the difference of HBV DNA level between HBe Ag positive HBe Ag negative pregnant women with chronic AsC,and to provide experimental basis for the establishment of individualized program of pregnancy care for pregnant women with chronic hepatitis B virus carriers and the prevention and treatment of mother-to-child vertical transmission of HBV.Methods:1.From September 2016 to January 2018 in the obstetrical outpatient clinic of Chenzhou NO.1 Hospital,151 pregnant women with chronic hepatitis B virus carriers and 141 pregnant women without hepatitis B were collected as according to the inclusion criteria of pregnant women with chronic hepatitis B virus carriers and pregnant women without hepatitis B,according to the results of hepatitis B serological examination,pregnant women with chronic hepatitis B virus carriers were divided into the pregnant women with HBe Ag negative chronic hepatitis B virus carriers group and HBe Ag positive chronic hepatitis B virus carriers group.2.Serum alanine aminotransferase(ALT)was detected by UV-lactate dehydrogenase method,Serum aspartate aminotransferase(AST)by malate dehydrogenase method,Serum Iron(Fe)by Ferrizine method,Serum ferritin(Fet)by turbidimetry;hepatitis B surface antigen(HBs Ag),Hepatitis B surface antibody(HBs Ab),hepatitis B e antigen(HBeAg),hepatitis B e antibody(HBeAb)and hepatitis B core antibodyby(HBcAb)were detected by time-resolved immuno fluorescence method.3.HBV DNA level was detected by Real-time fluorescence quantitative PCR(RT-PCR).4.HBV preC/BCP gene of samples were amplified by nested PCR,sequencing of PCR products,and then genotyping by Blast comparison.5.Graph Pad Prism 7.00 was used for statistical analysis of the experimental results.Results:1.The serum Fe,Fet levels of pregnant women with chronic AsC [17.20(9.30 ±23.20)μmol/L,36.40(12.20 ±92.50)μg/L] were higher than that of non-hepatitis B pregnant women [14.30(9.15 ±18.40)μmol/L,17.60(7.30 ±31.50)μg/L],the difference was statistically significant(P < 0.05,P < 0.05).2.The serum log10 HBV DNA level of pregnant women with HBe Ag positive chronic AsC was 7.86(7.31±8.21)IU/mL,which was significantly higher than that of HBe Ag negative chronic AsC pregnant women with 3.41(2.92 ±4.11)IU/mL,(P <0.05).3.In 151 cases of chronic AsC pregnant women,PCR amplification,sequencing and HBV genotyping were successful in 86 cases,type B accounted for 87.21%(75/86),type C accounted for 12.79%(11/86),no other genotypes were found.4.HBV genotype B chronic AsC pregnant women with high and low HBV DNA level accounted for 69.33%(52/75),30.67%(23/75),respectively,HBV genotype C chronic AsC pregnant women with high or low HBV DNA level accounted for 81.82%(9/11),18.18%(2/1),respectively,the difference was statistically significant(P <0.05).Conclusion:The serum HBV DNA load of pregnant women with chronic AsC is higher,the HBV genotype was mainly type B,and the serum HBV DNA level of HBe Ag positive chronic AsC pregnant women was higher than that of HBe Ag negative chronic AsC pregnant women.It is necessary to pay close attention to the serum HBV DNA load and hepatitis B serological index of pregnant women with chronic AsC in order to reduce the vertical transmission of HBV from mother to child. |
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