| OBJECTIVE:To explore the treating mechanism of Kuntai Capsule for tripterygium glycosides-induced premature ovarian insufficiency from the perspective of molecule.METHODS:1.Female SD rats were continuously given tripterygium polyglycosides tablets 50mg/kg for 14 days,and then rats with failed modeling were removed,the rest rats were randomly divided into model group,kuntai group,Chinese and western medicine group and western medicine group,the normal female SD rats were taken as the control group.The levels of E2、LH、FSH and AMH in the peripheral blood of rats were detected by ELISA,the pathological changes of the ovarian tissues of rats in each group were observed by HE staining,the expression of Smad2/3/7 in the ovarian tissues was detected by western blot and PCR.2.Compared with the normal group,the macroscopic observation of model group showed obvious ovarian tissue atrophy,and HE staining showed decreased follicles at all levels,damaged follicular structure,less and poorly developed corpus luteum.Compared with model group,drug intervention group has improved,visible antral follicle、primordial follicle、primary follicles、secondary follicles and mature follicle,visible egg cell、cumulus oophorus、zona pellucida、corona radiata,there were more granulosa cell layers around the follicle than in the model group,regularly arranged,the number of corpus luteum was more than that of model group,and developed fair.3.Compared with the normal group,the expression of Smad2/3 in the ovarian tissues of the model group was significantly decreased,while the expression of smad7 was increased,and the difference was statistically significant(P<0.05).Compared with the model group,except the expression of smad3 in the western medicine group showed no statistical significance,the expression of Smad2/3 in the kuntai group、the chinese and western medicine group and the western medicine group showed a significant difference(P<0.05).Compared with western medicine group,there were significant differences in smad3 expression between the kuntai group and the chinese and western medicine group(P<0.05).4.Compared with the normal group,the expression of Smad2/3 mRNA in the ovarian tissues of the model group was significantly decreased,while the expression of smad7mRNA was increased,and the difference was statistically significant(P<0.05).Compared with the model group,the expression of Smad2/3 mRNA in the kuntai group、the chinese and western medicine group and the western medicine group increased,while the expression of smad7mRNA decreased,and the difference was statistically significant(P<0.05).CONCLUSIONS:①The rat model of POI induced by tripterygium glycosides was established successfully;②Kuntai capsule could improve the sex hormone level of POI rats induced by tripterygium polyglycosides;③Kuntai capsule can repair tripterygium glycosides-induced premature ovarian insufficiency to some extent by regulating the expression of Smad2/3/7 in ovarian tissue,whose mechanism remains to be further studied. |
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