| Objectives 1 To investigate the effect of Bushen Tiaochong recipe on the incidence of premature ovarian failure induced by Tripterygium Glycosides tablets.2 Study the effect of Bushen Tiaochong recipe of premature ovarian failure in the prevention, treatment and intervention, and provide ideas for traditional Chinese medicine to prevent and treatment premature ovarian failure.Methods female SD rats were allocated randomly into six groups: blank group, model group,estrogen group(control group), Bushen Tiaochong recipe of prevention group;treatment group and intervention group. 10 rats in each group. Blank group were treated normal feeding. The model group were administrated by gastric perfusion of Tripterygium Glycosides tablets. The prevention group was treated with Bushen Tiaochong recipe before model building; the treatment group was treated with Bushen Tiaochong recipe at the same time as model building; the intervention group was treated with Bushen Tiaochong recipe after model building. The control group used conjugated estrogens aqueous gavage, the same way as Bushen Tiaochong recipe each group.According to serum sex hormone levels in rats, ovarian histomorphology,immunohistochemical methods, protein and gene detection of ovarian related cytokines as observations, to observed pharmacodynamic and prevention effects of Bushen Tiaochong recipe.Results 1 Effect of estrous cycle in rats: The rats in the blank group with regular estrous cycle, 4~5d/times.Estrous cycle of rats in the model group rested on anestrus for long time, 8~10d/times, which even without the normal estrous cycle. Compared with the blank group, there was significant difference between the prevention group with low-dose group and the model group(P<0.05). The treatment group with low, middle-dose group and model group compared with the blank group, there was a significant difference(P<0.05). The intervention of each group and control group compared with blank group, the difference was significant(P<0.05). 2 Influence of serum sex hormone levels in rats: 1)prevention group:The serum levels of E2, AMH, INHB were significantly higher than that of the model group(P<0.01). The content of LH and FSH was lower than that of model group, and there was significant difference(P<0.01). 2) Treatment group: The serum levels of E2, AMH, INHB were significantly higher than that of the model group(P<0.01). The content of LH and FSH was lower than that of model group, and there was significant difference(P < 0.01). 3) intervention group:The levels of E2, INHB content increased compared with model group, there was a significant difference(P<0.01); FSH, LH decreased compared with model group, there was a significant difference(P<0.01). Compared with the model group, the content of AMH in middle and high dose group and control group were significantly different(P < 0.01). 3 Influence of morphological change of ovarian tissue in rats: Bushen Tiaochong recipe of middle, highdose group and control group in prevention group,high-dose group and control group in treatment group showed each stage follicles, well-developed, no rats ovarian volume atrophy, reduced the number of follicles, and less granulosa layers. 4 Effect of Bcl-2, Bax,TNF-α, IFN-γ protein and mRNA expression in ovarian tissue in rats: 1) prevention group: Bushen Tiaochong recipe of each dose group and the control group Bcl-2 protein and mRNA expression were significantly lower than that of model group, there was a significant difference(P<0.01). 2) Treatment group: Bushen Tiaochong recipe of each dose group and the control group Bcl-2, Bax, TNF-α protein and mRNA expression compared with model group, there was a significant difference(P<0.01);. IFN-γ mRNA expression compared with model group decreased, there was a significant difference(P<0.01); The expression of IFN-γ in the middle, high-dose group and control group was lower than that of model group, and there was significant difference(P < 0.01). 3)intervention group: Each group bcl-2 protein and mRNA expression compared with model group increased, there was a significant difference(P<0.01); Bax, TNF-α protein and mRNA expression compared with the model group decreased, there was significant difference(P < 0.01); IFN-γ mRNA expression compared with the model group decreased, with significant difference(P < 0.01). In comparison, the middle,high-dose group and control group IFN-γ protein expression with model group decreased, there was significant difference(P<0.01).Conclusions In the rat model of premature ovarian failure in the manufacture of Tripterygium Glycosides tablets:1 The middle,high group of Bushen Tiaochong recipe has a protective effect on ovary, the same effect with conjugated estrogens tablets, can prevent the occurrence of premature ovarian failure. The mechanism may be some chinese herbal medicinal ingredients in Bushen Tiaochong recipe can regulate the level of ovarian hormones; increase the expression of Bcl-2, decrease the expression of Bax,maintain the balance of Bcl-2, Bax, reduce TNF-α and IFN-γ production rate and follicular atresia, to ensure the normal apoptosis in ovarian cells and maintain the normal physiological function of the ovaries.2 Bushen Tiaochong recipe can treat premature ovarian failure. The same efficacy with conjugated estrogens tablets. By increasing the sensitivity of ovarian sex hormones, promote ovulation; increase the expression of Bcl-2,decrease the expression of Bax, reducing levels of TNF-α and IFN-γ, thereby inhibiting excessive apoptosis of ovarian granulosa cells, reducing follicular atresia and promote the recovery of ovarian function.3 Bushen Tiaochong recipe can suppress the occurrence of premature ovarian failure. The results of this study suggest that the expression of the intervention group of hormone level and factors not only have differences with the control group, but also with model group. It showed that ovarian function did not fail, but at the stage of decline of ovarian reserve function. It shows that no premature ovarian failure but in a period of decline in ovarian reserve function, therefore reduce the degree of ovarian damage and improve the cure rate of ovarian damage.4 Giving Bushen Tiaochong recipe to prevent the POF is better than the simple treatment effect.Conclusion the tip: use of gonadal damage drugs for the treatment of diseases in clinic, we should use the advance has a protective effect on the gonads of drugs, such as Bushen Tiaochong Recipe, in order to make the gonads from harm or damage reduced to a minimum. |
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