The Effection Of Biological Function About Kiss-1 In Gastric Cancer Cell Line

Objective:To investgate the relationship between the expression of Kiss-1 and gastric cancer cell line MGC-803,and observe the ability of proliferation,migration and invasion of MGC-803 by using siRNA-Kiss-1（small interfering RNA of Kiss-1）to down-regulated the expression of Kiss-1,then observed the influence on the biological function of gastric cancer cell lines.Methods:1 The gastric cancer cell line MGC-803（poor differentiation）was cultured in CO₂ cell incubator for cytological experiment.2 The cell transfection was mediated by Lipofectamine 2000,which resulted in decreasing the expression of Kiss-1 of gastric cancer cell line MGC-803.3 Quantitative Real-time PCR（Real-time PCR）was used to detect mRNA expression in MGC-803 cells of transfected with siRNA-Kiss-1（si group）,siRNA-nc（negative control group）,and con（blank control,without transfection treatment）after 48 hours culture.4 Cell Proliferation Assays cells of con（control group）,siRNA-nc（negative control group）and siRNA-Kiss-1（experimental group）in 96-well plates were added with CCK-8,and absorbance was measured after 2-4 hours to draw the proliferation curve.5 Transwell assay was used to detect the migration and invasive ability in the cells among siRNA-Kiss-1 group,transfected siRNA-nc group and con group.6 Wound Healing was applied to detect the migration ability of siRNA-Kiss-1group,siRNA-nc group,and con group.Results:1 Kiss-1 was low expressed in siRNA-Kiss-1 group and high expressed in control group and siRNA-nc group after transfection.The difference between groups was significant（P<0.05）.2 CCK-8:When cultured the cells at 24h,48h,96h,the absorbance of MGC-803 cells in siRNA-Kiss-1 group was significantly lower than siRNA-nc group and con group（P<0.05）.3 Transwell Assays:The migrated and invaded cells of siRNA-Kiss-1group was significantly higher than that of siRNA-nc group and control group（P<0.05）4 Wound Healing:healing ability of siRNA-Kiss-1 group was higher than the control group and siRNA-nc group（P<0.05）Conclusion:1 CCK-8 proliferation experiments showed that the proliferation ability of MGC-803 cell was decreased after down-regulated Kiss-1,which suggested that Kiss-1 may not inhibit its proliferation ability.2 Transwell Assays detected that the migration and invasion ability of MGC-803 cells was increased when down-regulated the Kiss-1,which indicated that Kiss-1 may inhibit gastric cancer cells migration and invasion ability.3 Wound Healing showed that transfected with siRNA-Kiss-1,the migration ability of MGC-803 was more enhanced,which indicated that Kiss-1 inhibited its migration ability.4 The results of cell function experiment indicated that Kiss-1 has potential to become a new therapeutic target which inhibits gastric cancer migration and invasion ability.