Expression Of MMP-2 In Human Gastric Cancer And Effects Of Its Antisense Oligodeoxynucleotides On Growth And Invasion In Human Gastric Cancer SGC 7901 Cell Line In Vitro

Objective 1.To investigate the expression of matrix metalloproteinase 2(MMP-2)in human gastric cancer and its relationship with micro-angiogenesis and lymph node metastasis;2.To investigate the effects of MMP-2 antisense oligodeoxynucleotides on proliferation and invasion in gastric cancer cell line SGC7901.Methods 1.The expression of MMP-2 was detected by immunohistochemistry in 92 gastric adenocarcinomas and 21 normal gastric mucosas. Microvessel density (MVD) was detected by labeling vascular endothelial cells with CD34 and lymphatic microvessel density was detected by labeling lymphatic vessel endothelial cells with VEGFR-3;2.Transmit thiophosphorylate modified antisense oligodeo- xynucleotides of MMP-2 into gastric cancer cell line SGC7901 by liposome, MMP-2 mRNA levels were detected by reverse transcriptase-polymerase chain reaction(RT-PCR); changes of cell migration and invasion abilities in vitro were detected by transwell chamber invasion assay; cell proliferation was measured by methyl thiazolyl tetrazolium(MTT). Results 1.Positive rates of MMP-2,CD34 and VEGFR-3 in human gastric cancer tissues were higher than those in normal gastric mucosa.Expression of MMP-2 was related with histopathological grading,invasion range,lymph node metastasis and TNM stages(P<0.05).MVD was correlated with histopathological grading,invasion range,lymph node metastasis and TNM stages(P<0.05); VEGFR-3 was correlated with invasion range,lymph node metastasis.2.The expressions of MMP-2 mRNA in transfected gastric cancer cells significantly decreased. Treatment gastric cancer cells with MMP-2 ASODN inhibited the migration and invasion activities markly in a dose-dependent manner,however,no difference about cell proliferation was observed between experiment groups and control group in our study.Conclusions1.MMP-2 was significantly correlated with micro-angiogenesis and lymph node metastasis;2.The MMP-2 ASODN could significantly inhibit the expression of MMP-2 mRNA and migration and invasion activities in gastric cancer cell line SGC 7901 without affecting cell proliferation. So MMP-2 may become another target for the clinical treatment.