Pathological Study Of Lymph Node Metastasis,Clinical Pathological Features And Prognosis Of Oral Cancer

Objective:To evaluate oral squamous cell carecinoma(OSCC)in the department of oral and maxillofacial surgery in the Stomatological Hospital of Jilin University from 2006 to 2016.Selected 83 cases of oral squamous cell carcinoma,and observed the expression of MMP-14,Vimentin,E-cadherin,were detected by immunohistochemistry,and study the relationship between them and clinicopathological factors was analyzed,and the prognosis of oral cancer patients and the mechanism of metastasis were discussed.To study the expression of MMP-14,Vimentin,E-cadherin,VEGF and D2-40 in oral squamous cell carecinoma(OSCC)and to investigate the relationship between MMP-14,Vimentin E-cadherin VEGF,D2-40 expression and pathologic diagnosis,clinicopathogic featres and prognosis.Methods:The data of maxillofacial surgery in stomatological hospital of Jilin University were collected,and the clinical characteristics of 2006-2016 patients with oral squamous cell carcinoma treated in our hospital were analyzed.we tested the expression of MMP-14,Vimentin and E-cadherin by immunohistochemical in 83 cases of OSCC case and 20 in adjacent normal tissues,and combined with statistical analysis.Result:1.In 2006-2016 years,the overall volume of oral squamous cell carcinoma increased year by year.The ratio of male to female was 2.76:1,the average age of patients was 58.25 + 11.07 years old,and the proportion of tongue area was the highest(30.02%).Tissue of patients with oral squamous cell carcinoma with pathological staging in differentiated squamous cell carcinoma(%)is the most common,of which 620 cases surgery in patients with lymph node metastasis rate was24.68%(153/620),the total detection of 4712 lymph nodes,the lymph node metastasis number is 412,the average per node transfer positive patients with 0.66 lymph node involvement.2.The expression level of MMP-14 protein in the OSCC tissue was significantly higher than that in the surrounding normal tissue(P<0.01);In OSCC tissue,the MMP-14 expression were positively correlated with lymph node metastasis(P<0.01),low differentiated degree(P<0.05),and the T3-T4 tumor stage(P<0.05).MMP-14 showed significant positive correlation with Vimentin,and negative correlation with E-cadherin(P<0.01).3.The positive expression of mmp-14 and VEGF,as well as the increase of lymph node density and lymph node metastasis in patients,the positive rate of VEGF in mmp-14 positive patients was significantly higher than that in patients with mt1-mmp negative(88.89%/26.32%P<0.05),and the average of LVD in mmp-14 positive patients wassignificantly higher than that of mmp-14 negative patients(30.62± 7.402/17.74± 3.622 P<0.05),which was statistically significant.4.Of the 83 cases of oral cancer,44 patients died of this disease,and39 patients survived,the total survival rate was 46.99%.Among the 44 deaths,the expression of MMP-14 was significantly correlated with the patient’s survival(72.72%/27.27% P<0.001),and 35 cases of lymph node metastasis,of which MMP-14,VEGF,D2-40 were high.The expression(82.86%/17.14%,74.29%/25.72%,28.8 + 8.010)and the prognosis of patients with lymph node metastasis were significantly correlated(P<0.001),that is,the expression of MMP-14 and the high expression of VEGF and the high expression of D2-40(high density of lymphatic vessels)are closely related to the lymph node metastasis.Conclusion:1.Through to the jilin university stomatological hospital for nearly10 years of clinical data,according to a survey in our hospital patients with oral squamous cell carcinoma of the male than female patients,aged between 57.55±10.79 years old,and the predilection sites were the tongue,floor of mouth,gums,lips,cheeks and other parts.2.This study has found the close relationship between high expression of MMP-14 in oral carcinoma and the recurrence and prognosis.Epithelial-mesenchymal transition may promote invasion and metastasis of oral carcinoma,and MMP-14 promotes EMT.3.Mmp-14 is closely related to oral cancer lymphangiogenesis and lymph node metastasis,and is an important factor in the formation of lymphangiogenesis in oral cancer.