Inguinal Hernia Repaired By Bionic Human-derived Extracellular Matrix Mesh

destinations:In order to reduce the complications such as pain,infection or sperm cord injury caused by the clinical available undegradable meshes in tension-free inguinal hernia repair,We have developed human-deri-ved extracellular matrix mesh which would reduce the complications caused by undegradable meshes.The bionic mesh is a future trend.Ideal bionic inguinal hernia meshes should be bionic human-being tissue structure and components.It should also have the similar extracellular matrix components.In tissue,extracellular matrix(ECM)provides critical mechanical support for cells and leads cells to adhesion and growth.Xenogeneic ECM exerts potential immunogenicity,but human being ECM is not readily available.Xenogeneic extracellular matrix(ECM)exerts potential immunogenicity,but human being ECM is not readily available.Hepatic B Virus(HBV)can activate the hepatic stellate cells(HSCs)to enhance the secretion of extracellular matrix(ECM)which plays a key role for the development of liver cirrhosis.According to previous study,the activated HSCs presented a stronger ability in secretion of collagen than fibroblasts.Methods:We have prepared polycaprolactone-gelatin nanofibers scaffold with electrospinning,following the analysis for cell-compatibility,biocompatibility,immunogenic,mechanical strength and porosity.We seed HBV activated HSCs on the PCL-gelatin scaffold.The activated HSCs would secrete ECM such as collagen on the prepared scaffold which would be cultured under self-made bioreactor.The ECM nanofibers remodels under the stress,and crosslinked with PCL nanofibers.At intervals,the ECM was analyzed for an ideal production parameter.Then we removed the cellular components of the scaffold complex and developed a human-derived extra cellular derived mesh to replace the present polymer meshes so as to reduce the complications caused by the undegradable meshes.The prepared tissue-engineering inguinal hernia patches were implanted to the animal inguinal hernia models;at intervals,regular checking,samples collection and examinations including incision infection,histology,implanted cells tracing and spermatic cord function or structure will be performed.Results:This study for bionic human-derived ECM mesh provides practical theory and program,it has been confirmed that the patch prepared can form tissues in vivo,laid the theoretical foundation for the future bionic meshes into clinical application,and the meshes effects on the spermatic cord is small and does not cause adhesion.The meshes can offer support in the course of degradation process,under pressure in the abdominal cavity in each time point.Conclusions:the preparation of bionic hernia meshes to patch the clinical application,not only can complete the repair of hernia and abdominal wall that maintain complete function,but also cause small inflammation,and degraded gradually until the meshes have be completely replaced by self tissue finally.