The Correlation Research Between The Autophagy Level And Quantity Of CD₈⁺T Lymphocyte In Peripheral Blood In Patients With Chronic Obstructive Pulmonary Disease

Objective By detecting the differences in expression level of autophagy relevant genes and protein LC3 and p62,as well as the quantity and apoptosis of the CD₈⁺T lymphocyte in peripheral blood of Chronic Obstructive Pulmonary Disease（COPD）patients in catabasis,so as to explore the correlation research between the autophagy level and the quantity of CD₈⁺T lymphocyte.To explore the causes of the increase of CD₈⁺T cells in patients with COPD in catabasis,which it is helpful to clarify the pathogenesis of COPD.Methods There are three groups,which contain COPD group,smoking with normal lung function group（SN group）,and non-smoking with normal lung function group（NSN group）,each group contains 30 cases.Using FCW to detect the ratio of CD₈⁺T cells,CD₈⁺effector T cells and CD₈⁺memory T cells as well as the apoptosis rate of them.Sorting out CD₈⁺T cells in Trizol by flow cytometry（FCW）,using real-time quantitative PCR（qRT-PCR）and Western Blot to detect the expression level of CD₈⁺T lymphocytes autophagy relevant genes LC3,p62 in peripheral blood.Results 1.Flow cytometry detection shows:（1）The quantity of CD₈⁺T cells in COPD group（32.33±4.23%）was higher than the other two groups,and the results were statistically significant（P<0.05）.The quantiiest of both CD₈⁺effector T cells（25.63±8.57%）and CD₈⁺memory T cells（11.94±5.77%）were higher in COPD group than the other two groups（P<0.05）.The number of CD₈⁺T cells,CD₈⁺effusive T cells and memory T cells in the SN group were higher than those in NSN group（p<0.05）.（2）The apoptosis rate（23.51±6.11%）of CD₈⁺T lymphocytes in COPD group was lower than those in the other two groups（P<0.05）,while the rate in SN group was lower than that in NSN group（p<0.05）.2.there was no significant difference among the groups in expression level of gene LC3（P>0.05）.However,the expression levels of gene P62 was higher in COPD group than the SN normal group（p<0.05）,while the level in SN group was higer than the NSN group（p<0.05）.3.The expression level of LC3Ⅱ/Ⅰin COPD group was higher than those in SN group（p<0.05）,while the level in SN group was higher than that in NSN group.As to P62,the expression level in COPD group was higher than that in SN group（p<0.05）,while the SN group was higher than the NSN group（p<0.05）.4.Pearson correlation analysis showed that within the COPD group,the expressing level of LC3Ⅱ/Ⅰhas a positive correlation with the quantity of CD₈⁺T cells（r=0.572,p<0.05）,and CD₈⁺effector T cells（r=0.672,p<0.05）,and CD₈⁺memory T cells（r=0.739,p<0.05）.There was no correlation between the expression leve of LC3Ⅱ/Ⅰand the apoptosis rate of CD₈⁺T cells.（2）.The expression level of P62 had a positive correlation with the quantity of CD₈⁺T cells（r=0.613,p<0.05）,and CD₈⁺effector T cells（r=0.681,p<0.05）,and CD₈⁺memory T cells（r=0.745,p<0.05）.The expression level of P62 was not significantly correlated with the apoptosis rate of CD₈⁺T cells.5.regression analysis showed that within the COPD group,the linear connection between LC3Ⅱ/Ⅰ/GAPDH（‰）and the quantity of CD₈⁺T cells,and the quantity of CD₈⁺effective T cells,and the quantity of CD₈⁺memory T cells were all statistically significant（P<0.001）.（2）the linear connection between P62/GAPDH（‰）and the quantity of CD₈⁺T cells,and the quantity of CD₈⁺effective T cells,and the quantity of CD₈⁺memory T cells were all statistically significant（P<0.001）.Conclusions 1.The quantity of CD₈⁺T cells,CD₈⁺effector T cells and CD₈⁺memory T cells in peripheral blood of COPD patients in stable phase all increased significantly,while the apoptosis rate of them were lower.2.The expressing level of autophagy correlation protein LC3Ⅱ/Ⅰand P62 were significantly increased in the stable phase of COPD patients.3.The increased autophagy level and the increased quantity of CD₈⁺T cells had a positive correlation and linear connection,which suggest that the cell autophagy join in the pathogenesis of COPD.