Effects Of Bufei Yishen Granules Combined With Acupoint Sticking Therapy On Immunoglobins And T Lymphocyte Subsets In Rats With Chronic Obstructive Pulmonary Disease

Objective 1 To observe the effects of Bufei Yishen granules combined with “Shu Fei Tie” acupoint sticking therapy on pulmonary function and morphormetry based on the COPD model.2 To explore the effects of Bufei Yishen granule combined with “Shu Fei Tie” acupoint sticking therapy on immunoglobulins in peripheral blood and bronchoalveolar lavage fluid as well as T lymphocyte subsets.Methods Seventy-two rats were randomized into control group(CG), model group(MG), Bufei Yishen group(BYG), acupoint sticking group(ASG), Bufei Yishen + acupoint sticking group(BY+ASG), and aminophyline group(AG). COPD rats were duplicated by repeated cigarette smoke and bacterial exposures. The rats were treated with normal saline, Bufei Yishen granules, acupoint sticking, Bufei Yishen + acupoint sticking and aminophyline respectively from week 9 to week 20. Pulmonary function was detected by using whole body plethysmograph every 4 weeks. The rats were sacrificed at the end of the 20 th week. Lung tissue histology was observed under light microscope. Ig G, Ig A and Ig M in peripheral blood and BALF were detected by immunoturbidimetry method. T lymphocyte subsets in peripheral blood and BALF were detected by flow cytometry(FCM).Results 1 General status Since week 4, rats in model group were emaciated, depressed, inactive, less food and water intake. The symptoms were gradually improved after treatment, especially in BYG and BY+ ASG. At week 0, there were no significantly statistical difference between the rats(P>0.05). At week 8, the body weight gains of rats in MG weresignificantly lower than CG(P<0.01). At week 20, the body weight and body weight gains in MG were significantly lower than CG(P<0.01), and that in the treatment groups were significantly higer than MG(P<0.01). There was no significantly statistical difference among the treatment groups(P>0.05). 2 Pulmonary function From week 4, PEF and EF50 in MG decreased compared with CG(P<0.01, P<0.05). From week 8, TV in MG was lower than CG(P<0.01). At week 16, TV in BYG, ASG, BY + ASG and AG was higher than MG(P <0.01, P<0.05), while EF50 in BYG and BY + ASG was higher than MG(P<0.01, P<0.05). At week 20, TV, PEF, EF50 in BYG and BY + ASG increased compared with MG(P<0.01, P <0.05), while EF50 in ASG and TV in AG were higher than MG(P <0.05). 3 Pulmonary histopathology There was non apparent abnormality in CG. Lung histology was impaired obviously in MG. In BYG, ASG, BY+ASG and AG, it was alleviated at different levels, especially in BYG and BY+ASG. 4 Serum levels of Ig A, Ig G and Ig M Ig A, Ig G and Ig M in MG significantly decreased compared with CG(P<0.01). Compared with MG, Ig A, Ig G and Ig M in the treatment groups were higher(P<0.05, P<0.01). Ig G in BYG was higher than AG(P<0.05). Ig A, Ig G in BY+ASG were higher than ASG and AG(P<0.05, P<0.01). Compared with BY+ASG, Ig G in BYG was higher(P<0.05). 5 BALF levels of s Ig A, Ig G and Ig M s Ig A, Ig G and Ig M significantly decreased in MG compared with CG(P<0.01). Compared with MG, s Ig A and Ig G significantly increased in the treatment groups(P<0.01), and Ig M in BYG and BY+ASG increased(P<0.05, P<0.01). s Ig A and Ig G in BY+ASG were higher than AG(P<0.05, P<0.01), while Ig G was higher than BYG(P<0.01). 6 T lymphocyte subsets in peripheral blood CD3+, CD4+ and CD4+/CD8+ significantly decreased in MG compared with CG(P<0.01), while CD8+ increased(P<0.01). Compared with MG, CD4+/CD8+ was higher in the treatment groups(P<0.05,P<0.01), while CD8+ was significantly lower(P<0.01). CD4+ was significantly higher in BYG and BY+ASG than MG(P<0.01) and CD3+ increased in BY+ASG compared with MG(P<0.05). Compared with AG,CD4+ and CD4+/CD8+ were higher in BYG(P<0.05). CD3+, CD4+ and CD4+/CD8+ were higher in BY+ASG than AG(P<0.05, P<0.01), but CD8+ was lower(P<0.05). Compared with ASG, CD4+ and CD4+/CD8+ increased in BY+ASG(P<0.05, P<0.01) while CD8+ decreased(P<0.05). 7 T lymphocyte subsets in BALF CD3+,CD4+ and CD4+/CD8+ significantly decreased in MG compared with CG(P<0.01), while CD8+ increased(P<0.01). Compared with MG, CD4+ and CD4+/CD8+ were significantly higher in the treatment groups(P<0.01), while CD8+ was lower(P<0.01, P<0.05). CD3+ was higer in BYG and BY+ASG than MG(P<0.01). CD4+, CD4+/CD8+ in BYG increased compared with AG(P<0.05). CD4+ and CD4+/CD8+ were higher in BY+ASG than ASG and AG, while CD8+ were lower(P<0.05). CD4+ and CD4+/CD8+ were higher in BY+ASG than BYG(P<0.05).Conclusion 1 There are beneficial effect of Bufei Yishen granule combined with “Shu Fei Tie” acupoint sticking therapy in the treatment of stable COPD, which can significantly improve symptom, lung function, and reduce the pathological damage in lung tissue. 2 Bufei Yishen granule combined with “Shu Fei Tie” acupoint sticking therapy can significantly improve the levels of immunoglobulins and T-lymphocyte subsets in rats with COPD, thus improve the immune function.