| Objectives To observe the effect of acupuncture on the clinical efficacy of women undergoing in vitro fertilization-embryo transfer(IVF-ET)and frozen-thawed embryo transfer(FET)again and explore the application value of acupuncture in assisted reproductive technology.Methods Selected from June 2016 to January 2018 in Tangshan Maternal and Child Health Hospital Department of Reproductive Genetics,133 cases of patients undergoing embryo transfer again.Long-acting gonadotrophin-releasing hormone agonist(Gn RH-a)long-term protocol was used in 100 patients with IVF-ET cycle,47 patients in acupuncture group and 53 patients in control group.After entering the cycle,the acupuncture patients began to receive Feiteng Bafa combined with the routine acupuncture method of selecting points according to the pattern identification since the end of menstruation,and began to acupuncture at Shenque at the time of ovulation induction,acupuncture treatment to the day before transplanting day(no acupuncture at the day of egg retrieval).The control group did not undergwent acupuncture intervention.Compare the dosage and time of gonadotropin(Gn),follicle output rate(FORT),the endometrial thickness,morphology,the levels of serum estradiol(E2),luteinizing hormone(LH),follicle-stimulating hormone(FSH)and progesterone(P)on the day of injection of human chorionic gonadotropin(HCG),oocyte count,MII eggs count,fertilized eggs count,splitting cells rate,transplantable embryo rate,high-quality embryo rate,cycle outcome and embryo cultivation rate,clinical pregnancy rate were observed in the two groups patients.33 patients with FET cycles were treated with artificial cycles,including 13 in the acupuncture group and 20 in the control group.The acupuncture patients began to receive acupuncture treatment started 7 days after ovulation in the previous menstrual cycle to the day before transplanting day(methods are as the same as those in the IVF-ET acupuncture patients.Menstrual period is not acupuncture).The control group did not undergo acupuncture intervention.Compare the difference between the two groups of patients with estrogen dosage,days of medication,endometrial thickness and morphology,embryo cultivation rate and clinical pregnancy rate.Results 1 There were no significant differences in age,infertility period,body mass index(BMI)and basic hormone levels between the IVF-ET cycle acupuncture group and the control group(P>0.05).2 The differences were not significant in Gn does,Gn medication time,FORT between the experimental group and the control group of IVF-ET cycle(P>0.05).3 The differences in endometrial thickness on HCG day were not significant between the groups of IVF-ET cycle(P>0.05).The A type endometrial proportion on HCG day in the observation group was higher then those in the control group(87.23% vs 69.81%),B type endometrial proportion was lower then those in the control group(12.77% vs 30.19%),indicating the significant difference(P<0.05).4 There were no significant differences in the levels of serum E2,LH,FSH and P on HCG day between the experimental group and the control group of IVF-ET cycle(P>0.05).5 The differences were not significant in oocyte count,MII eggs count,fertilized eggs count between the experimental group and the control group of IVF-ET cycle(P>0.05).In the experimental group,the splitting cells rate was higher than that in the control group(96.12% vs 91.12%),the difference was statistically significant(P<0.05).6 The differences were not significant in transplantable embryo rate,cycle outcome and embryo cultivation rate,clinical pregnancy rate between the experimental group and the control group of IVF-ET cycle(P>0.05).The rate of high-quality embryos in the acupuncture group was more than that in the control group,and the difference was statistically significant(P<0.05).7 There were no significant differences in the amount of estrogen,the number of medication days,the thickness and morphology of the endometrial between the experimental group and the control group of the FET cycle(P>0.05).8 The differences were not significant in embryo cultivation rate and clinical pregnancy rate between the experimental group and the control group of the FET cycle(P>0.05).Conclusions 1 Acupuncture treatment can improve the endometrial morphology,and improve the endometrial receptivity to some extent.2 Acupuncture treatment can increase the quality of eggs,and improve the splitting cells rate,promote embryonic development and increase the rate of high-quality embryos in the IVF-ET process.It can be considered as an adjunctive treatment for infertile patients with poor endometrial morphology,poor quality of eggs and embryos. |
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