Effect Of Bushen Tiaochong Recipe On SCF And Cx37 In Rats With Premature Ovarian Failure Induced By Tripterygium Wilfordi

Objectives To investigate the effect of Bushen tiao Chong Fang on ovarian function injury in rats with premature ovarian failure and the changes of the expression of ovarian related stem cell factors and gap connexin.Methods Female SD rats were randomly divided into blank group,model group,estrogen model group,drug injection group(high,medium and low doses),drug group after estrogen making,and 10 rats in each group(high,medium and low doses).In addition to normal feeding in blank group,the rats in other groups were established by 75mg/kg perfusion with Tripterygium Glycosides tablets.The rats’ estrous cycle and behavior change were observed.The serum sex hormone levels were detected by enzyme linked immunosorbent assay,and the changes of the tissue structure of the rat ovary were observed under light and electron microscope.The protein and gene expression of the ovarian related factors SCF and Cx-37 in rats were detected by immunohistochemistry and RT-PCR method.The experimental data were analyzed with SPSS 21 statistics software,and the results of the quantitative data between the two groups were expressed in S.The quantitative data were compared with the single factor analysis of variance,and the difference P < 0.01 or P < 0.05 was statistically significant.Results 1 Bushen tiao Chong Fang has certain preventive and therapeutic effects on premature ovarian failure in rats,which can repair ovarian function damage,reduce follicular atresia,and restore normal physiological function of ovary.2.The change of hormone level: the level of sex hormone in the model group was disordered,and the hormone levels were significantly different in the model group and the model group(P < 0.05).3.The effect on the histomorphology of the rat ovary: under the light microscope,the oestrogen group and the high dose group of the Chinese medicine did not see the atrophy of the ovary,and the follicles were well developed at all levels,but the number of the follicles decreased and the follicle granula matured.Under the electron microscope,the nucleus space of the ovary in the model group was widened,the nucleoplasm shifted and the mitochondria vacuolated.The crista lysis disappeared and some rough endoplasmic reticulum appeared degranulation.However,there was no obvious abnormality in the organelles of ovarian cells in the high / middle dose group and the combined estrogen group,and the nucleolus was large and clear.4.The expression of protein and mRNA in the rats with premature ovarian failure: the content of SCF protein and mRNA in the model group was increased and the content of Cx-37 protein and mRNA decreased.The expression of SCF,Cx-37 protein and mRNA in the rat model was different from that of the model group(P < 0.05),and the expression level of Cx-37 and SCF in the high dose group and the estrogen group was not significantly different from those in the blank group.P > 0.05),which was significantly different from the middle and low dose group(P < 0.05).Conclusions 1 Bushen tiao Chong Fang has some preventive effect on premature ovarian failure.It can reduce follicle atresia and maintain normal physiological function of ovary.2 The high-dose Bushen Tiaochong high-dose group had a better therapeutic effect than the middle and low-dose group,and was equivalent to the combined estrogen tablet group.It increased ovarian sensitivity to gonadotropin and promoted ovarian ovulation;it was down-regulated by up-regulating Cx-37 expression.The expression of SCF promotes the growth and development of early follicles,improves the gap junctional function of rat ovarian tissue,and repairs the damage of ovarian function.